Evaluation of antimicrobial compounds from Juniperus communis L. and Vitex negundo L. against Xanthomonas sp. and Pseudomonas sp.
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Date
2015
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YSPU
Abstract
Essential oils from the shade dried leaves of Juniperus communis L. and Vitex negundo L. from H.P. (India) were examined for their antimicrobial activities against eight human and plant pathogenic bacteria. Essential oils were obtained by using Soxhlet extraction apparatus. Chemical analysis of the oils was done by using gas chromatography and mass spectrometry (GC/MS). The antimicrobial effects were tested by inhibition zone diameter (IZD)/ zone of inhibition test. Essential oil from methanol extract showed very good
activity at 300 ppm concentration after 72 hours and was more effective as compared to others, however, all the plant extracts showed inhibitory effect on linear growth of selected microorganism. Higher concentrations of 1-Phenanthrene carboxylic acid (11.44 %) and Propylparaben, Benzoic acid, 4-hydroxy, Benzoic acid, 3-hydroxy and Benzoic acid, 3- hydroxy-2-methylpropyl ester (23.23%) content released from Juniperus communis L. and Vitex negundo L. H.P. (India) respectively, might be assigned to be responsible for such
excellent antimicrobial activity against the tested pathogens. Docking studies were also performed for sixteen compounds in order to evaluate their affinity to bacterial proteins that are known targets for some antibiotics with different mechanisms of action. The purpose of the present studies was to encourage use of Juniperus communis L. and Vitex negundo L. essential oils against bacterial infections so as to provide an approach to develop herbal formulations as potential disease management strategies in place of chemicals. However, the
results are yet to be authenticated through wet lab experimentations for each biomolecules before going ahead for potential drug development.
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Keywords
extraction, oils, bacteria, essential oils, planting, alcohols, land management, organic compounds, concentrates, solvents