ISOLATION AND CHARACTERIZATION OF ENDOPHYTIC BACTERIA AND THEIR SYNERGISTIC EFFECT ON GROWTH PERFORMANCE OF BELL PEPPER (Capsicum annuum L.)
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Date
2019-08-03
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UNIVERSITY OF AGRICULTURAL SCIENCES GKVK, BENGALURU
Abstract
Endophytes are the microorganisms (fungi or bacteria) that lives inside the plant
tissue symbiotically. They can be promoting plant growth by producing phytohormones,
siderophores and enhancing resistance to plant pathogens. The study was conducted to
isolate, characterize and screen for efficient endophytic bacteria from Bell pepper plant
samples collected from Department of Horticulture, Gandhi Krishi Vigyan Kendra and
IIHR, Hesaragatta Bengaluru. Total thirty bacterial endophytes were isolated from Bell
pepper leaves, stems and roots. These endophytic bacterial isolates were subjected to
various morphological and biochemical test. The identified genera were Pseudomonas and
Bacillus. Among thirty endophytic bacterial isolates three efficient isolates were selected
based on their higher efficiency in N fixation, P solubilization, Siderophore and IAA
production. The effect of selected efficient isolates (CPL-3, CPS-8 and CPR-4) and one
reference strain was Bacillus megaterium were tested single and dual inoculation in nine
treatments and three replications under pot culture experiment. The plant growth
parameters like plant height, number of leaves, number of branches and number of flowers
per plant and yield parameters like number of fruits, diameter of fruits and fruit yield per
plant and also nitrogen, phosphorous and potassium content of plants were found highest
in the ninth treatment involving RDF + Pseudomonas isolate (CPL-3) + Pseudomonas
isolate (CPS-8) + Bacillus isolate (CPR-4) + FYM.