Evaluation of pyrolyzed pine needle bio-oil for antifungal and nematicidal activities with molecular docking analysis on target proteins

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dc.contributor.advisorVerma, A. K.
dc.contributor.authorChandra, Kailash
dc.date.accessioned2022-12-20T14:41:08Z
dc.date.available2022-12-20T14:41:08Z
dc.date.issued2022-09
dc.description.abstractPyrolyzed pine needle bio-oil formulation was used to evaluate antifungal and nematicidal activity. Antifungal activity of pine needle bio-oil (40%) based formulation and its four dilutions (10-1, 10-2, 10-3 and 10-4) were evaluated in both condition in-vitro and in-vivo. In-vitro activity of the formulation and its 10-1 showed 100% inhibitory activity against Alternaria brassicae while in 10-2, 10-3 and 10-4 dilutions the inhibitory activity was 52%, 48% and 42% respectively. The pre-conditioning brassica plants treated with the foliar spray of antifungal pine needle bio-oil formulation showed 100% inhibition of Alternaria brassicae while in post-conditioning plants treated with dilutions 10-1, 10-2, 10-3 and 10-4 were showed disease severity of about 3.3%, 6.6%, 13.3% and 30% respectively. Biochemical analysis of pine needle bio-oil formulation treated Brassica leaves revealed that the chlorophyll and carbohydrates content of post-conditioning plants increased with respect to the increase in bio-oil dilutions while protein content was found to be decreased. In case of post-conditioning plants, only protein content was increased while the chlorophyll and carbohydrates content was found to be similar. Molecular docking analysis of four compounds present in pine needle bio-oil, 4-methyl-pentanoic acid and nonanoic acid with succinate dehydrogenase while 1-(1H-Imidazol-4-YL)-Ethanone and dehydroabietic acid with lanosterol 14α demethylase of Alternaria brassicae. Showed dock score -4.03 and -6.9 Kcal/mol for the enzyme succinate dehydrogenase and -9.6 and -6.8 Kcal/mol for lanosterol 14α demethylase respectively. Nematicidal activity of pine needle bio-oil based formulation was analyzed against root knot nematode Meloidogyne spp. The concentration of formulation 5%, 7.5% and 10% showed maximum 100% inhibition against mortality, immobility and egg hatching of Meloidogyne spp. Molecular docking analysis of four compounds present in pine needle bio-oil, dehydroabietic acid and 2-methyl phenol with thymidylate synthase while dehydroabietic acid and 4-Methyl Pentanoic acid with Na-K ATPase of Meloidogyne spp. Showed dock score -7.1 and -4.94 Kcal/mol for thymidylate synthase and -5.0 and -4.8 Kcal/mol for Na-K ATPase respectively. Hence the pine needle bio-oil based formulation is more effective against Alternaria brassicae nematode Meloidogyne sppen_US
dc.identifier.urihttps://krishikosh.egranth.ac.in/handle/1/5810190705
dc.keywordsbio-oil, pine needle, antifungalen_US
dc.language.isoEnglishen_US
dc.pages83en_US
dc.publisherG.B. Pant University of Agriculture and Technology, Pantnagar, District Udham Singh Nagar, Uttarakhand. PIN - 263145en_US
dc.research.problemBio-oilen_US
dc.subBiochemistryen_US
dc.themePine needleen_US
dc.these.typeM.Scen_US
dc.titleEvaluation of pyrolyzed pine needle bio-oil for antifungal and nematicidal activities with molecular docking analysis on target proteinsen_US
dc.typeThesisen_US
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