Biodegradation of Zenzo (α) Pyrene by native bacteria employing nanoparticles
Loading...
Date
2021-02, 2021-02
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand)
Abstract
In present study, potential of microorganisms to degrade a high molecular weight polyaromatic hydrocarbon viz. Benzo(α)Pyrene (BaP) has been investigated. Three bacteria isolated from coal tar contaminated and fire affected soils were tested for their BaP degrading ability in minimal medium and soil slurry. Based on their morphological, biochemical and molecular characterization, the bacterial isolates were identified as Cellulomaons sp. 4D, Commamonas sp.1B and Streptomyces sp. 2C. All the isolates performed best at pH 7, temperature 30° C and shaking speed 100 rpm. Supplementing the minimal medium with additional carbon and nitrogen sources enhanced the rate of BaP degradation by the bacterial isolates. Comparative studies showed that BaP degradation potential of consortium is better than individual bacteria. Viability of bacterial cells has also been tested by storing them in bioformulation made of charcoal and talc and in alginate beads for 60 days. BaP degradation was also investigated using bacterial isolates immobilized in sodium alginate beads and using nanoparticles in both minimal medium and soil. Results showed that the degradation potential of bacterial isolates increased when used in immobilized form. Nanoparticles enhanced the ability of bacterial isolates to degrade BaP in soil though much difference was not observed in minimal medium. HPLC and FTIR analysis confirmed the degradation of BaP by the selected bacterial isolates. The current study clearly demonstrated that Cellulomaons sp. 4D, Commamonas sp. 1B and Streptomyces sp.2C were able to degrade BaP effectively in media and soil and therefore can be used profitably for field scale bioremediation technology