Mutational Analysis of Nucleocapsid (N) Protein of Newcastle Disease Virus Using a Minigenome System

Simple item page
dc.contributor.authorVidhya, M
dc.contributor.authorJohn Kirubaharan, J
dc.contributor.authorRajalakshmi, S
dc.contributor.authorRajasekaran, Ranjini
dc.contributor.authorTANUVAS
dc.date.accessioned2020-03-05T10:24:58Z
dc.date.available2020-03-05T10:24:58Z
dc.date.issued2020-02
dc.descriptionTNV_20thMVC_PP_Feb-2020_OP48en_US
dc.description.abstractThe Nuclepcapsid protein (N) protein of Newcastle disease virus (NDV), synonymous with Avian Avula virus-1(AAv-1) functions primarily to encapsidate the virus genome for transcription, replication and packaging. In addition to its role in replication, N gene is the major immunogenic and conserved gene of NDV. Identifying mutation permissive regions in the N protein provides a potential approach for NDV marker vaccine. In the present study mutations introduced in to the NP gene in the form of epitope replacement between amino acid positions 446-453 was analysed using an intracellular NDVD58-GFP minigenome assay for its effect on minigenome RNA synthesis and encapsidation.Site directed mutagenesis was carried out in pCIneo N plasmid to replace the NDV epitope 446(QFLDLMRAV)453 with a marker epitope TAVSPTTLR.The mutated pCIneo N plasmid was co-transfected with other NDV expression plasmids pCIneo L, pCIneo P and pNDVD58-GFP minigenome plasmid in BSR/T7 cells. GFP expression was observed 72 hours post transfection indicating a functional minigenome. Further the presence of encapsidated minigenome RNA in transfected cell supernatants were also confirmed by Real time PCR. These results indicate that the mutations did not affect the functionality of the Nucleoprotein and can serve as marker epitopes for NP based ND marker vaccine.en_US
dc.identifier.urihttp://krishikosh.egranth.ac.in/handle/1/5810144282
dc.keywords20th Indian Veterinary Congress, Annual Conference of IAAVR, TANUVASen_US
dc.language.isoenen_US
dc.pages1en_US
dc.subjectVeterinary Scienceen_US
dc.titleMutational Analysis of Nucleocapsid (N) Protein of Newcastle Disease Virus Using a Minigenome Systemen_US
dc.title.alternativeNational Symposium on Veterinary Research Priorities in Translational Animal Health, Production and Food Safetyen_US
dc.typePresentationen_US
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
TNV_20thMVC_PP_Feb-2020_OP48.pdf
Size:
96.45 KB
Format:
Adobe Portable Document Format
Description:
TNV_20thMVC_PP_Feb-2020_OP48
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
Poster Presentations