Studies on plant growth promoting bacteria from jeevamrit, a liquid bioformulation

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dc.contributor.advisorSaini, Raj
dc.contributor.authorVaishali
dc.date.accessioned2022-10-28T10:21:25Z
dc.date.available2022-10-28T10:21:25Z
dc.date.issued2021-10-28
dc.description.abstractThe present investigation entitled “Studies on plant growth promoting bacteria from jeevamrit, a liquid bioformulation” was carried out during 2021 with the aim to study the plant growth promoting (PGP) potential of bacterial isolates in jeevamrit samples collected from three sites each at two different locations (Nadaun, Hamirpur) of district Hamirpur of Himachal Pradesh. The pH, N, P and K contents of the six jeevamrit samples ranged between 4.87-7.66, 1.49-2.65%, 0.004-0.052% and 0.059-0.111%, respectively. The culturable bacterial count on nutrient agar medium varied between 1.76×107 to 10.45×107 cfu/ml of jeevamrit. Preliminary screening of 112 bacterial isolates from jeevamrit revealed 66.96, 16.96, 66.96, 8.93 and 7.14% isolates to show phosphate solubilization, potassium solubilization, nitrogen fixation, siderophore production and ammonia production, respectively. Only 63.39, 27.68, 39.29, 33.04 and 38.39% isolates displayed antifungal activity against phytopathogens viz. Alternaria sp., Fusarium sp., Sclerotium sp., Sclerotinia sp. and Colletotrichum sp., respectively. Out of 112, eighteen best isolates were selected, and none displayed hydrogen cyanide production and only nine isolates showed phytase activity. Sixteen of 18 isolates showed inhibition of all phytopathogens with maximum percent inhibition (43.75-61.44%) recorded for isolate NJA-13. The phosphate solubilization index (1.69 to 12.25) on Pikovskaya’s agar was recorded highest for isolate HJA-21. The phosphate solubilization (50.25-476.50 μg/ml) in NBRIP liquid medium containing tricalcium phosphate was found maximum in isolate JJA-8. Bacterial isolates successfully produced indole-3-acetic acid (0.15 to 27.40 μg/ml), highest produced by CJV-10. Of the eighteen isolates, only 16.67, 44.44, 38.89, 5.56 and 50% isolates exhibited amylase, gelatinase, protease, cellulase and lipase, respectively. The four promising bacterial isolates NJA-13, HJA-21, JJA-8 and CJV10 with multiple PGP traits were identified as Bacillus subtilis strain NJA-13 (Acc. No. OK036919), Levilactobacillus brevis strain HJA21 (Acc. No. OK036950), Paucilactobacillus vaccinostercus strain JJA8 (Acc. No. OK036952) and Bacillus safensis strain CJV 10 (Acc. No. OK036956), respectively, on the basis of 16S rRNA gene technology. Seed bacterization with six bacterial isolates showed significant enhancement of percent seed germination, root and shoot length, and vigour index with most isolates as compared to control. Overall, the study revealed jeevamrit as a consortium of beneficial bacteria that can be utilized as potential biofertilizers and biocontrol agents, and hence, reflects the beneficial effects of application of jeevamrit in agriculture for crop production and protection.en_US
dc.identifier.urihttps://krishikosh.egranth.ac.in/handle/1/5810189431
dc.keywordsJeevamrit, Bacterial isolates, Antifungal activityen_US
dc.language.isoEnglishen_US
dc.pages103+iven_US
dc.publisherCollege of Horticulture and Forestry Neri, Hamirpur (H.P.)en_US
dc.subSoil Sciencesen_US
dc.themePromoting bacteria from Jeevamriten_US
dc.these.typeM.Scen_US
dc.titleStudies on plant growth promoting bacteria from jeevamrit, a liquid bioformulationen_US
dc.typeThesisen_US
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