OPTIMIZATION OF MICROPROPAGATION PROTOCOL OF SANDAL (Santalum album L.).
| dc.contributor.advisor | Gahukar, Dr. S. J. | |
| dc.contributor.author | WASHIMKAR, HARIDAS BALIRAM | |
| dc.date.accessioned | 2018-07-25T10:42:30Z | |
| dc.date.available | 2018-07-25T10:42:30Z | |
| dc.date.issued | 2017-10-10 | |
| dc.description | Micropropagation provide opportunity in order to develop reliable and rapid micropropagation protocol and to produce disease and virus free plantlets, to standardize explant type and media combinations amenable for tissue cultureto in vitro micro propagation of Santalum album. | en_US |
| dc.description.abstract | Present investigation aimed, to produce disease and virus free plantlets, to standardize explant type and media combinations amenable for tissue culture, to in vitro micro propagation of Santalum album was conducted in Completely Randomized Design at Centre of excellence in Plant Biotechnology, Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Akola, Dist. Akola (M.S.) during the academic year 2015-2017. Survival percentage of nodal explants after two and three week’s treatment involving MS + BAP 1mg/l + kinetin 1mg/l recorded highest survival percentage of 70.00% and 68.33percent respectively. For initiation of shoot bud after two weeks the nodal explants were inoculated on MS + BAP + Kin @ 1mg/l The highest percent of survival was recorded i.e.63.33 percent. After three weeks of inoculation on MS + BAP + Kin @ 1mg/l each the highest percent of shoot bud induction was i.e. 61.66 percent. For multiple shoot induction after three and six weeks treatment involving MS + BAP 3 mg/l + kin 1 mg/l + ADS 2mg/L highest survival of multiple shoot were recorded, in three weeks (5.06) and after six weeks (2.81). For Seed germination when MS 3 % without GA3 and MS 3%, 4%, and 6% with GA3 1mg/l was tried the highest seed germination was observed (86.66) when MS 3%+ GA3 1mg/l was used. The explants derived from in vitro grown seedlingexplants were (shoot, epicotyl, hypocotyl) inoculated on 8 different media combination.The highest survival percentage was recorded in treatment MS + BAP 2mg/L + NAA 0.4 + IBA 0.4% in case of shoot (91.66%), epicotyls (50%), and hypocotyl (33.33%). shoot explants shoot performed better followed by epicotyls and hypocotyl. The in vitro germinated seedlings are transfer on media combinations of BAP 1mg/l with IBA 0.2, 0.4 and IAA 0.4, 0.4. The highest percent of survival of seedling was observed on ½ MS liquid, MS + BAP 1 mg/l + IBA 0.2 mg/l and MS + BAP 1 mg/l + IBA 0.4 mg/l and MS + BAP 1 mg/l + IBA 0.4 mg/l (100 percent.) | en_US |
| dc.identifier.citation | WASHIMKAR, HARIDAS BALIRAM. (2017).Optimization of micropropagation protocol of sandal (Santalum AlbumL.)Department of Agricultural Botany, Dr. Panjabrao Deshmukh Krishi Vidyapeeth,M. Sc. 2017. Print. xiii, 68p. (Unpublished). | en_US |
| dc.identifier.uri | http://krishikosh.egranth.ac.in/handle/1/5810062000 | |
| dc.keywords | Forest crop, Sandal, Santalum album, Crop improvement, Biotechnology, Tissue culture, Plant physiology, Kinetin GA3 Indol Butaric acid, Indol acetic acid, Napthalic acetic acid, Micropropagation, Sandal, Biotechnology, Tissue culture, Explants, Plant growth regulators, | en_US |
| dc.language.iso | en | en_US |
| dc.pages | xiii, 68p. | en_US |
| dc.publisher | Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Akola, Maharashtra. | en_US |
| dc.research.problem | The researcher has tried to study on standardization of parameter for in- vitro propagation of sandal and standardization of micro propagation parameters for explants obtained from in-vitro germinated seedling of sandal. The researcher has also tried to study Comparison of in-vitro propagation method with traditional method of propagation. | en_US |
| dc.sub | Agricultural Biotechnology | en_US |
| dc.subject | null | en_US |
| dc.theme | To produce disease and virus free plantlets, within short duration. | en_US |
| dc.these.type | M.Sc | en_US |
| dc.title | OPTIMIZATION OF MICROPROPAGATION PROTOCOL OF SANDAL (Santalum album L.). | en_US |
| dc.type | Thesis | en_US |