IN- VITRO REGENERATION AND TRANSFORMATION OF PATCHOULI (Pogostemon cablin Benth.), WITH A REPORTER GENE AND ITS IN-VIVO PROPAGATION
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Date
2023-03-27
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University of Agricultural Sciences, Bangalore
Abstract
Patchouli (Pogostemon cablin. Benth.) is one of the important aromatic plants mainly cultivated for its essential oil. The oil blends well with essential oils and imparts strength, character and alluring notes. The oil is used in the manufacture of perfumery chemicals, food flavours and flavouring of other consumer products. With the growing demand for its oil an attempt for an effective and reproducible in vitro regeneration protocol for patchouli, its transformation with reporter genes and effect of growth regulators on in-vivo propagation is performed to determine the influence of different growth regulators on rooting of patchouli cuttings for quick and successful multiplication. Patchouli successfully regenerated in SIM (MS media with 2.0mg/ L of BAP and 0.1 mg/L of NAA) and RIM of 0.5 mg/ L IAA, for all parameters recorded and was used as the media for its transformation. The plant was transformed with GUS and GFP genes and are confirmed with histochemical and PCR assays. Among the growth regulators, combination of IBA and NAA (3000 ppm) recorded highest percentage of rooting (83.75 %), more number of sprouts (22.05), early sprouting (15.88 days) and root initiation (10.50 days), maximum root length (15.25 cm), and highest survival percentage (91.50 %) and was on par with IBA at 3000 ppm. IBA at 5000 ppm recorded highest length of sprouts (18.63 cm) on par with NAA at 3000 ppm (18.60 cm). Combination of IBA and NAA each at 3000 ppm is found to be the best treatment for propagation of patchouli.