ISOLATION AND IDENTIFICATION OF BACTERIAL AGENTS ASSOCIATED WITH POLYARTHRITIS IN CALVES

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dc.contributor.advisorPriya P. M
dc.contributor.authorNAIR ASWATHY
dc.date.accessioned2020-09-23T05:42:51Z
dc.date.available2020-09-23T05:42:51Z
dc.date.issued2019
dc.description.abstractBlood and synovial fluid/wound swabs were collected from thirty one calves with polyarthritis presented to University Veterinary Hospitals, Mannuthy and Kokkalai, organised and unorganised farms of Thrissur district to identify the bacterial etiology. Out of 18 bacterial isolates obtained, 12 were Gram- positive and six were Gram-negative bacteria. Based on cultural, morphological and biochemical tests, they were identified as Staphylococcus aureus (38.9 per cent), S. epidermidis (11.1per cent), Streptococcus spp. (11.1 per cent), Corynebacterium jeikeium (5.6 per cent), Escherichia coli (5.6 per cent), Klebsiella spp. (5.6 per cent), Pseudomonas aeruginosa (5.6 per cent) and Salmonella spp. (5.6 per cent). Polymerase chain reaction (PCR) was standardised using designed primers for Corynebacterium spp. and Staphylococcus spp. for their confirmation. One and nine E. coli isolates were obtained from the wound and rectal swabs respectively. The identity of the E. coli isolated were confirmed by pho A based PCR. On screening against virulence genes like lt, fim H, k99, f41, stx1, stx2, eaeA, cnf1, cnf2, ehlyA, cdt III and f17. E. coli isolated from rectal swabs were positive for stx1, eae A, cnf1 and cdt III. Whereas all genes were negative for the E.coli isolated from wound swab. On genus specific PCR, none of the DNA extracted from the synovial fluids were positive for mycoplasmal infection. Gentamicin was found to be the most effective drug against the isolates obtained. The blood parameters revealed leukocytosis, thrombocytosis and granulocytosis. Increase in total protein and leucocytosis were observed on synovial fluid analysis.en_US
dc.identifier.urihttps://krishikosh.egranth.ac.in/handle/1/5810151813
dc.keywordsISOLATION IDENTIFICATION BACTERIAL AGENTS ASSOCIATED POLYARTHRITIS CALVESen_US
dc.language.isoEnglishen_US
dc.pages112en_US
dc.publisherCollege of Veterinary and animal Science,Mannuthyen_US
dc.research.problemISOLATION AND IDENTIFICATION OF BACTERIAL AGENTS ASSOCIATED WITH POLYARTHRITIS IN CALVESen_US
dc.subVeterinary Microbiologyen_US
dc.themeISOLATION AND IDENTIFICATION OF BACTERIAL AGENTS ASSOCIATED WITH POLYARTHRITIS IN CALVESen_US
dc.these.typeM.V.Sc.en_US
dc.titleISOLATION AND IDENTIFICATION OF BACTERIAL AGENTS ASSOCIATED WITH POLYARTHRITIS IN CALVESen_US
dc.typeThesisen_US
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