ISOLATION AND IDENTIFICATION OF BACTERIAL AGENTS ASSOCIATED WITH POLYARTHRITIS IN CALVES
Loading...
Date
2019
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
College of Veterinary and animal Science,Mannuthy
Abstract
Blood and synovial fluid/wound swabs were collected from thirty one calves
with polyarthritis presented to University Veterinary Hospitals, Mannuthy and
Kokkalai, organised and unorganised farms of Thrissur district to identify the
bacterial etiology. Out of 18 bacterial isolates obtained, 12 were Gram- positive and
six were Gram-negative bacteria. Based on cultural, morphological and biochemical
tests, they were identified as Staphylococcus aureus (38.9 per cent), S. epidermidis
(11.1per cent), Streptococcus spp. (11.1 per cent), Corynebacterium jeikeium (5.6
per cent), Escherichia coli (5.6 per cent), Klebsiella spp. (5.6 per cent),
Pseudomonas aeruginosa (5.6 per cent) and Salmonella spp. (5.6 per cent).
Polymerase chain reaction (PCR) was standardised using designed primers for
Corynebacterium spp. and Staphylococcus spp. for their confirmation. One and
nine E. coli isolates were obtained from the wound and rectal swabs respectively.
The identity of the E. coli isolated were confirmed by pho A based PCR. On
screening against virulence genes like lt, fim H, k99, f41, stx1, stx2, eaeA, cnf1,
cnf2, ehlyA, cdt III and f17. E. coli isolated from rectal swabs were positive for stx1,
eae A, cnf1 and cdt III. Whereas all genes were negative for the E.coli isolated from
wound swab. On genus specific PCR, none of the DNA extracted from the synovial
fluids were positive for mycoplasmal infection. Gentamicin was found to be the
most effective drug against the isolates obtained. The blood parameters revealed
leukocytosis, thrombocytosis and granulocytosis. Increase in total protein and
leucocytosis were observed on synovial fluid analysis.