MOLECULAR CHARACTERIZATION OF TOMATO MOSAIC VIRUS AND CUCUMBER MOSAIC VIRUS INFECTING TOMATO (Solanum lycopersicum L.) AND INDUCTION OF DEFENSE USING BIOMOLECULES
| dc.contributor.advisor | NAGARAJU, N. | |
| dc.contributor.author | ASHWINI, B.N. | |
| dc.date.accessioned | 2023-01-03T03:52:54Z | |
| dc.date.available | 2023-01-03T03:52:54Z | |
| dc.date.issued | 2022-03-23 | |
| dc.description.abstract | Tomato mosaic virus (ToMV) and cucumber mosaic virus (CMV), are considered as the most important virus diseases infecting tomato. The characterized ToMV-To-Kar isolate genome had 6380 nucleotides and shared 97.00- 99.56 per cent nucleotide identity with other ToMV isolates/strains. Tripartite CMV-To-Kar isolate comprised of RNA1 3360 nt), RNA2 (3050 nt) and RNA3 (2217 nt) shared 92.00-98.58, 91.12 -98.84 and 92.03 - 98.23 per cent nucleotide identity with subgroup IB of CMV respectively. Among 20 different biomolecules tested for induced defense, least mean ToMV disease severity (3.33 %) was observed in plants primed with milk and Simarouba glauca 5.00%)correspondingly with higher defense enzyme activities i.e., PO (2.10 and 4.95 fold), PPO (3.06 and 1.74 fold) and PAL (1.45 and 1.37 fold) and with 3.59 and 3.22 fold increased accumulation of SA, respectively. Similarly, a great reduction in CMV disease severity was observed in S. glauca and milk i.e., 100.0 and 87.50 per cent respectively. They recorded enhanced PO (4.57 and 2.67 fold), PPO (1.47 and 3.05 fold) and PAL (1.13 and 1.17 fold) activities with 3.82 and 3.29 fold change accumulation of SA, respectively. The defined biomolecules showed restrictive activity of Myzus persicae with relative deterrence index between 0-1 in detached leaf assay, whole plant assay and also least number of aphids in leaf disc assay. Out of 35 entries screened for resistance against ToMV and CMV under glasshouse conditions, TLB-409-1-2, LA3924 and Sankranti showed resistant and moderately resistant reaction to CMV and ToMV respectively. | en_US |
| dc.identifier.other | Th-13189 | |
| dc.identifier.uri | https://krishikosh.egranth.ac.in/handle/1/5810191022 | |
| dc.keywords | CUCUMBER, TOMATO, MOSAIC, VIRUS INFECTING TOMATO | en_US |
| dc.language.iso | English | en_US |
| dc.pages | 227 | en_US |
| dc.publisher | University of Agricultural Sciences, Bangalore | en_US |
| dc.sub | Plant Pathology | en_US |
| dc.theme | CUCUMBER MOSAIC VIRUS INFECTING TOMATO | en_US |
| dc.these.type | Ph.D | en_US |
| dc.title | MOLECULAR CHARACTERIZATION OF TOMATO MOSAIC VIRUS AND CUCUMBER MOSAIC VIRUS INFECTING TOMATO (Solanum lycopersicum L.) AND INDUCTION OF DEFENSE USING BIOMOLECULES | en_US |
| dc.type | Thesis | en_US |