COAT PROTEIN DIVERSITY IN BEAN COMMON MOSAIC VIRUS AND ELUCIDATION OF R-GENES IN INDIAN LANDRACES OF Phaseolus Vulgaris L.
Loading...
Date
2010
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
CSK Himachal Pradesh Krishi Vishavavidyalaya, Palampur
Abstract
Present investigations on bean mosaic disease caused by bean common mosaic virus (BCMV), a member of family Potyviridae were undertaken to study the molecular diversity different strains of the virus using 3’ terminal region and to elucidate the presence of R-genes to find out durable sources of resistance. Symptomatology of different isolates belonging to 4 strains viz., NL-1, NL-1n, NL-7 and NL-7n consisted of mosaic, blistering, downward leaf rolling, green vein banding, thickening of leaves, reduction of leaf lamina and leaf deformation. DAS-ELISA and RT-PCR analysis of test isolates established the identity of the virus as BCMV and their reaction on International differential set grouped them in 4 strains. RT-PCR generated an amplicon of ~1300 bp in different isolates whose cloned product after sequencing revealed that it consisted of 3’ terminal region having partial NIb, complete cp and 3’UTR. Sequences were submitted in NCBI genbank and their sequence homology, multiple alignment and evolutionary divergence analysis showed that this region could not differentiate the pathogenically variable strains of the same virus. Ninety one accessions of common bean were found resistant to NL-1 and NL-1n strain. Prediction of resistance genes using tightly linked markers showed the presence of I, bc-12 and bc-3 gene in resistant cultivars.
Description
Doctoral Diseases
Keywords
##Unable to generate tags d3086.pdf, Plant diseases, ##Unable to generate tags d3086.pdf