Bacterial strain improvement for biomolecules through chemical and physical mutagens

Simple item page
dc.contributor.advisorSingh, Dharam
dc.contributor.authorTHAKUR, AKRITI
dc.date.accessioned2022-02-03T09:12:58Z
dc.date.available2022-02-03T09:12:58Z
dc.date.issued2021-03-30
dc.description.abstractMicroorganisms are crucial life form present on earth, inhabiting all climatic zones including high altitude Himalayan niches. Microbes can thrive in harsh environmental conditions due to their ability to produce biomolecules such as enzymes and metabolites that perform specialised biological functions. Biomolecules produced by microbes are in minute quantities. Therefore, there is a need to increase the production for large scale applications through strain improvement. Hence, the current study was focussed on the strain improvement of unique bacterium Iodobacter sp. PCH194 through the application of chemical mutagens MMS, EMS, and NMU and physical mutagen in the form of UV radiation. The isolate PCH194 coproduces PHA and violacein, which has wide industrial applications. Through systematic applications of mutagens on wild-type PCH194, mutants with desired features were obtained and designated as IN1, IN2, IN3, IN4, and IN5. Their growth kinetics at alleviated temperature were observed. It was found that their growth temperature increased from 20 to 25C, and slow growth was also observed at 28C. However, the application of thermo protectants glycine betaine and glutamate could not significantly enhance the growth at 28C. There was a marked increase in growth, PHA and violacein production of the mutants at 20C. The PHA production was 1.24 mg/ml for IN5 and violacein production was 1.63 mg/ml for IN2, whereas wild strain produced 0.42 mg/ml PHA and 0.20 mg/ml violacein, respectively. In conclusion, the present study successfully increased the growth temperature of Iodobacter sp. PCH194 from 20C to 25C and also enhanced the production of PHA and violacein. Hence, generated mutants can further be used for process optimisation and scale-up studiesen_US
dc.identifier.urihttps://krishikosh.egranth.ac.in/handle/1/5810181629
dc.keywordsMicrobes,physical mutagen, chemical mutagen, industrial productionen_US
dc.language.isoEnglishen_US
dc.pages63en_US
dc.publisherPalampuren_US
dc.subAgricultural Biotechnologyen_US
dc.themeGeneration of mutants using physical and chemical mutagenesis and Evaluation of mutants for their biological activity.en_US
dc.these.typeM.Scen_US
dc.titleBacterial strain improvement for biomolecules through chemical and physical mutagensen_US
dc.typeThesisen_US
Files
Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
AkritiThakur Msc. Agr.Bio-tech..pdf
Size:
1.47 MB
Format:
Adobe Portable Document Format
Description:
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
Theses