STUDIES ON THE COMPARATIVE EVALUATION OF DIFFERENT EXTRACTION METHODS AND STANDARDIZATION OF HPLC CONDITIONS FOR ESTIMATION OF ACTIVE CONTENTS IN SOME COMMERCIALLY IMPORTANT MEDICINAL PLANTS
| dc.contributor.advisor | SHARMA, YASH PAL | |
| dc.contributor.author | SHARMA, SEEMA | |
| dc.date.accessioned | 2017-09-05T07:24:25Z | |
| dc.date.available | 2017-09-05T07:24:25Z | |
| dc.date.issued | 2017 | |
| dc.description.abstract | ABSTRACT The present investigation, “Studies on the comparative evaluation of different extraction methods and standardization of HPLC conditions for estimation of active contents in some commercially important medicinal plants” was carried out in the department of Forest Products, Dr Y S Parmar University of Horticulture and Forestry, Nauni, Solan, (H.P.) during 2013-2016. The study was conducted on five important medicinal crops viz., Swertia chirayita, Podophyllum hexandrum, Picrorhiza kurroa, Gloriosa superba and Andrographis paniculata with the objectives as (i.) Standardization of extraction technique, (ii.) Development and validation of HPLC method for quantification of active phytoconstituent(s). For standardization of extraction technique in each crop five different extraction methods viz., soxhlet extraction, reflux extraction, cold extraction, sonication assisted extraction and microwave assisted extraction were used with two different solvents. The extracting solvents and extraction durations vary with conditions. In Swertia chirayita maximum amaroswerin (0.242%) and amarogentin (0.294%) content with higher total extract (17.756%) were obtained under soxhlet extraction for eight hours with methanol solvent. In Podophyllum hexandrum, reflux extraction for four hours with methanol solvent is the best extraction method for extraction of maximum (5.794%) podophyllotoxin content with higher total extract (38.826%). Cold extraction with continous shaking at room temperature with methanol for twelve hours is the best extraction method for extraction of maximum picroside-I (8.600%) and picroside-II (3.834%) with total extract (39.430%) in Picrorhiza kurroa. In Gloriosa superba, maximum colchicine content and total extract was obtained under soxhlet extraction for one hour with methanol solvent or reflux extraction with ethyl alcohol for one hour. For Andrographis paniculata, reflux extraction for one hour with methanol solvent has been found best extraction method for obtaining maximum (2.040%) andrographolide content with total extract (15.111%). Simple and easy to handle HPLC methods were developed and validated for quantification of major phytoconstituents in medicinal crops under study. Each method was validated as per ICH guidelines for seven parameters viz., linearity, range, accuracy, precision, LOD, LOQ and robustness. | en_US |
| dc.identifier.uri | http://krishikosh.egranth.ac.in/handle/1/5810030452 | |
| dc.keywords | medicinal plants,extraction methods , standardization of HPLC conditions, active contents | en_US |
| dc.language.iso | en | en_US |
| dc.pages | 253+XVII | en_US |
| dc.publisher | UHF,NAUNI | en_US |
| dc.sub | Forest Products | en_US |
| dc.subject | null | en_US |
| dc.theme | medicinal plants,extraction methods , standardization of HPLC conditions, active contents | en_US |
| dc.these.type | Ph.D | en_US |
| dc.title | STUDIES ON THE COMPARATIVE EVALUATION OF DIFFERENT EXTRACTION METHODS AND STANDARDIZATION OF HPLC CONDITIONS FOR ESTIMATION OF ACTIVE CONTENTS IN SOME COMMERCIALLY IMPORTANT MEDICINAL PLANTS | en_US |
| dc.type | Thesis | en_US |