STUDIES ON IN VITRO PROPAGATION OF GISELA-5 (Prunus cerasus X Prunus canescens) - CHERRY ROOTSTOCK
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Date
2015
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Abstract
ABSTRACT
In the present investigation, a technique for in vitro propagation of Gisela-5 (Prunus cerasus x
Prunus canescens)- cherry rootstock has been developed. It was observed that maximum in vitro
establishment was achieved during the month of July and February. Treatment with 0.1 per cent
HgCl2 for 5 minutes was found to be the best as it gave maximum number of uncontaminated
buds and bud survival. Maximum in vitro establishment of explants (70%) was achieved on MS
medium fortified with 0.5 mg/l BA and 0.5 mg/l GA3. Highest multiplication rate of 1:5 was
observed on five medium combinations (MS medium + 0.3 mg/l BA + 0.2 mg/l GA3, MS+ 0.5
mg/l BA + 0.5 mg/l GA3 + 0.1 mg/l IBA, MS +0.3 mg/l BA + 0.2 mg/l GA3 + 0.1 mg/l IBA, MS
+ 0.25 mg/l BA + 0.1 mg/l IBA + 0.25 mg/l kin and MS + 0.3 mg/l BA + 0.5 mg/l GA3 + 0.1 mg/l
IBA). Shoot multiplication rate and shoot length showed an increase with the increase in number
of passages which increased to a maximal of 1:9 and 6 cm after third and fourth passage. Best
rooting of 18.20 per cent was observed after fourth subculture on half strength MS medium with
0.5 mg/l IBA in one step procedure whereas, in two step procedure of rooting, maximum rooting
(53.33%) was observed after 24 hours dark incubation in half strength MS broth fortified with 0.5
mg/l IBA followed by transfer to semisolid half strength MS basal medium. Rooted plantlets were
transplanted in sterilized sand for hardening and kept in the glasshouse. 90 per cent survival was
observed after 4 weeks of transfer. In vitro mother cultures were indexed for CLRV, ACLSV and
PNRSV using DAS-ELISA procedure. All the tested samples showed negative results for the
presence of these viruses.