Expression of pluripotency markers in buffalo (Bubalus bubalis) embryonic stem cell like cells

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dc.contributor.authorPalanisammi, A
dc.contributor.authorSatheshkumar, S
dc.contributor.authorVinoth, M, et al.,
dc.contributor.authorTANUVAS
dc.date.accessioned2021-11-30T10:56:44Z
dc.date.available2021-11-30T10:56:44Z
dc.date.issued2020
dc.descriptionTNV_JEZS_2020_8(6)1090-1093en_US
dc.description.abstractThe objective of present study was to culture and characterize the buffalo embryonic stem cell-like cells (BESC) for their pluripotency by analysing Oct-4 and TRA-1-81 markers. Blastomeres from two, four, eight, sixteen, morula and blastocyst of in-vitro produced buffalo preimplantation embryos were derived and were seeded on mitomycin inactivated buffalo foetal fibroblasts feeder layer and cultured. The cultured blastomeres were monitored colony formation. The colonies exhibiting typical morphological features of BESC were subcultured. Marker expressions were tested on passages 1, 2, 3 and 4 by RTPCR, immune-cytochemistry and flowcytometric analysis. RT-PCR study revealed that expression of Oct-4 progressively declined with subsequent passages. The BESC strongly reacted with TRA-1-81 antibodies. In flowcytometry, the cells expressing TRA-1-81 in the first passage and second passage were 98.03±0.03 per cent and 97.13±0.07 per cent respectively. However, in the third and fourth passages the expression levels were reduced to 12.11±0.04 and 4.21±0.09 per cent respectively. Thus the study proved that cultured BESC had pluripotency characters and from third passage they started differentiation process.en_US
dc.identifier.urihttps://krishikosh.egranth.ac.in/handle/1/5810178458
dc.keywordsBuffalo, embryonic stem cell, pluripotency markers, RT-PCR, flow cytometryen_US
dc.language.isoEnglishen_US
dc.pages1090-1093en_US
dc.relation.ispartofseries;6
dc.subjectVeterinary Scienceen_US
dc.titleExpression of pluripotency markers in buffalo (Bubalus bubalis) embryonic stem cell like cellsen_US
dc.title.alternativeJournal of Entomology and Zoology Studiesen_US
dc.typeArticleen_US
dc.volume8en_US
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