Biochemical investigation for functionally active principles of quality Brassica genotypes and profiling of seed storage protein
| dc.contributor.advisor | Punetha, Himanshu | |
| dc.contributor.author | Pujari, Rohit | |
| dc.date.accessioned | 2021-11-20T06:24:20Z | |
| dc.date.available | 2021-11-20T06:24:20Z | |
| dc.date.issued | 2021-10 | |
| dc.description.abstract | The family Brassicaceae possess different range of varieties that finds importance in medicine, agriculture, nutraceuticals, etc. Its health promoting factors including essential amino acids, β-carotene, flavonoids and other phytoconstituents has tremendous impact on health and hence can be used as a viable food ingredient. It possess some antinutrients like glucosinolates and phytic acid that restricts its use for human consumption. The present study includes the investigation of various constituents in the fifteen samples of various Brassica genotypes and the profiling of seed storage protein through SDSPAGE. The total antioxidant was recorded highest to be in sample Biochem2020-12 (22.092±0.0122 mg/g) while minimum was recorded to be in Biochem2020-6 (12.09±0.048), the highest content of tryptophan was observed in sample Biochem2020-04 (1.257±0.014 g/100g protein) while the minimum was observed to be in Biochem2020-03 (0.749±0.019 g/100g protein), the highest value of methionine was observed to be in sample Biochem2020-05 (2.177±0.011 g/100g protein) while the minimum was observed to be in Biochem2020-3 (1.852±0.038g/100g of protein) , the highest TPC was recorded to be in sample Biochem2020-15 (5.467±0.067 mg/g) while the minimum was recorded to be Biochem2020-6 (2.686±0.037 mg/g) , the ortho-dihydric phenol content was recorded to be highest in sample Biochem2020-08 (0.546±0.002 mg/g) while the minimum was recorded to be in Biochem2020-03 (0.287±0.001 mg/g), the total protein content observed among all the genotypes was recorded to be highest in Biochem2020-03 (34.77%) whereas lowest was recorded in Biochem2020-04 (31.51%). The highest oil content was observed to be in Biochem2020-12 (44.58%) while the lowest was observed in Biochem2020-09 (35.75%). The glucosinolate content was observed to be highest in sample Biochem2020-13 (81.23±1.002 μmol/g) while the minimum was observed in sample Biochem2020-03 (14.08±0.122 μmol/g). Biochem2020-6 proved to be a better anti-inflammatory agent having IC50 of 35.770±6.555μg/ml. The DPPH activity was best shown by sample Biochem2020-08 having percentage inhibition of 52.987±0.462 %. The reducing power of sample Biochem2020-04 having IC50 57.443 ±0.070 μg/ml was observed to be better among all the genotypes under investigation. The results were recorded with respect to quality check, PDZ-1. The protein profiling through SDS-PAGE resulted in the band pattern ranging from 14.3kDa to 40kDa. | en_US |
| dc.identifier.uri | https://krishikosh.egranth.ac.in/handle/1/5810178187 | |
| dc.keywords | biochemistry, crop quality, Brassica, genotypes, profiling, seed proteins, storage quality | en_US |
| dc.language.iso | English | en_US |
| dc.pages | 110 | en_US |
| dc.publisher | G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand) | en_US |
| dc.research.problem | Brassica | en_US |
| dc.sub | Biochemistry | en_US |
| dc.theme | Genotypes | en_US |
| dc.these.type | M.Sc | en_US |
| dc.title | Biochemical investigation for functionally active principles of quality Brassica genotypes and profiling of seed storage protein | en_US |
| dc.type | Thesis | en_US |