INVESTIGATION ON STONY PIT DISEASE OF PEAR

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dc.contributor.advisorHANDA, ANIL
dc.contributor.authorPUSHAP, RAJ
dc.date.accessioned2017-05-12T07:35:53Z
dc.date.available2017-05-12T07:35:53Z
dc.date.issued2014
dc.description.abstractABSTRACT Surveys conducted during 2013 in pear orchards of Kullu, Mandi and Shimla districts of Himachal Pradesh revealed the incidence of stony pit disease ranging from 10 to 65 per cent. Prominent symptoms associated with different cultivars were leaf vein yellowing, chlorotic leaf spots, fruit deformities with formation of pits, corky tissue beneath the pits and misshapen fruits alongwith russeting on the surface of fruits. Serological detection through DAC and DAS-ELISA resulted in the detection of apple stem pitting virus (ASPV) and apple chlorotic leaf spot virus (ACLSV) either alone or in combination in all samples of eight cultivarsmarked during survey. Biological detection of one of the isolate (Red Bartlett) containing infection of ASPV and ACLSV on herbaceous hosts resulted in the production of symptoms on Chenopodium amaranticolor, C. quinoa, Nicotiana glutinosa, N. tabacum and a few other hosts. Biological detection on woody indicators (Quince A, Quince B-29 and Quince C) resulted in the development of typical viral symptoms in all the indicators by double grafting method. Leaf and petal samples drawn during April-May and fruits during August were found suitable for the detection of ASPV and ACLSV in DAS-ELISA. Association of ASPV with viral symptoms in pear was also confirmed by RT-PCR. DAS-ELISA based serological screening resulted in the selection of 16 plants of seven cultivars free from ASPV infection.en_US
dc.identifier.urihttp://krishikosh.egranth.ac.in/handle/1/5810011052
dc.keywordspears, Mandi and Shimla districts of Himachal Pradesh,stony pit diseaseen_US
dc.language.isoenen_US
dc.publisherUHF,NAUNIen_US
dc.subPlant Pathologyen_US
dc.subjectnullen_US
dc.themepears, Mandi and Shimla districts of Himachal Pradesh,stony pit diseaseen_US
dc.these.typeM.Scen_US
dc.titleINVESTIGATION ON STONY PIT DISEASE OF PEARen_US
dc.typeThesisen_US
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