MOLECULAR MAPPING AND LOCALIZATION OF STABLE QTLs FOR CHARCOAL ROT RESISTANCE IN SORGHUM {Sorghum bicolor{L,) Moench)
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Date
2007-08-30
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University of Agricultural Sciences GKVK, Banglore
Abstract
The genetic architecture of charcoal rot resistance and drought
tolerance through the construction of molecular linkage maps and
identification of QTLs can expectedly hasten the development of charcoal
rot and drought tolerant sorghum cultivars. A set of 93 Recombinant
Inbred Lines (RlLs) derived from 1822380 and E36-1 were evaluated for
charcoal rot incidence and its related traits in sick plot at Main
Agricultural Research Station, Dharwad and Regional Agricultural
Research Station, Bijapur during rabi 2004. The RlLs displayed highly
significant differences in their mean performance for all traits.
A total of 240 RAPD, 262 genomic SSR and 20 genie SSR markers
were used and of which 19 RAPD, 62 genomic SSRs and 4 genie SSR
markers were found to be polymorphic between parents. All 85 markers
genotyped across RlLs and a genetic linkage map was constructed by
using MAPMAKER/EXP b 3.0 in which 80 markers were assigned to A, B,
C, D, E, F, G, H, 1 and J linkage groups. A total genomic length of 650.3
cM was covered in this map. QTL analysis by composite interval mapping
using QTL Cartographer (v2.5) indicated five and four QTLs for charcoal
rot component traits at Dharwad and Bijapur locations respectively on A,
B, D and 1 linkage groups. Three QTLs, one each for length of infection
[xtxp297 on B), number of internodes crossed by the fungus (AC13 on A)
and per cent lodging (xtxp343) explaining a phenotypic variance of 9.29,
12.54 and 15.2 per cent respectively were detected consistently between
two locations and are considered to be the stable across environments.
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