Interaction of Mungbean Yellow Vein Mosaic virus and Rhizobium Sp. in Mungbean [Vigna radiata (L.)
| dc.contributor.advisor | Mahatma, Lalit | |
| dc.contributor.author | Chudasama, Mitesh Khimjibhai | |
| dc.date.accessioned | 2018-07-17T11:51:14Z | |
| dc.date.available | 2018-07-17T11:51:14Z | |
| dc.date.issued | 2014-07 | |
| dc.description.abstract | Yellow Mosaic Disease of the mungbean caused by a variant MYMV-Vig: IN: NVS: Mg: 2012 in the South Gujarat. Rhizobium sp. is a symbiotic nitrogen fixing bacteria remain associated with the legumes. The present research work was aimed to find the interaction of Rhizobium sp. and MYMV for the exploiting the mechanism to reduce the disease and improve the yield and productivity. Ten different samples were collected to isolate the Rhizobium strain of mungbean. Through the isolation and initial biochemical characterization. three isolates (NAURh-l. NAURh·2 and NAURh-7) out of the ten isolates were suspected to be the Rhizobium sp. They • I showed white, round, convex, glistening, mucoid and opaque colonies on YEMA. These isolates showed negative reaction in IMViC test, H 2 S test, starch hydrolysis test, caesin hydrolysis test, nitrate reduction test, Gelatin hydrolysis test, whereas positiveAbstract reaction in oxidase and urea hydrolysis test, All the three isolates , look alike, therefore, only one isolate NAURh-2 was promoted for the further research. Among the different antibiotic tested, Sparfloxacin and Tetracycline were placed in the categories of ch loram phen ico I. intermediate; co-tri moxazo Ie, levofloxacin, ofloxacin, cefotaxime, gentamycin, tobramycin and streptocycline were susceptible oxacillin, • and • Im'penem, ciprofloxacin, meropenem, cafalaxin, cephalothion , moxifloxacin, amoxyclav, penicillin, ampicillin, erythromycin, vanomycin, clindamycin and sulphatriad were of resistant categories. Through the Biolog study and subsequently manual analysis of the data and biological studies it was concluded that the isolate (NAURh-2) was Rhizobium iegllmil1osarllm . Cell length of the bacteria varied from 1.2 to 3.0 I'm, whereas cell width varied from 0.5 to 1.0 I'm . The cells from the freshly prepared culture were Gram negative and found regular rod shaped. Cells were either singly or arranged in aggregates. No spore formation was observed even on the old and stressed cultures. Bacteria produces capsule were motile with peritrichous flagella . Nodulation in the crop could be seen at the 12 days onwards after the inoculation. Maximum 28 nodules per plant were observed at 32 post inoculation thereafter , it started decreasing in the present situation. Detail steps of the nodulation were studied. Significantly higher nodules were observed in the plants inoculated from the Rhizobium ieguminosarllm treatment, seed in the control treatment with condition. the Among Rhizobilllll the different iegllmil10sarumAbslraCI IOmllkg was found significantly superior. No effect ~f the early nodulation on the Rhizobium sp . nodulation and vice versa was observed. If this would have been observed as per the few published literature, this would have been exploited for the minimization of losses caused by the MYMV and enhancement of productivity of the high demanded protein source of vegetarian. | en_US |
| dc.identifier.citation | CHUDASAMA MITESH KHIMJIBHAI_64804 | en_US |
| dc.identifier.uri | http://krishikosh.egranth.ac.in/handle/1/5810060634 | |
| dc.keywords | Plant Pathology | en_US |
| dc.language.iso | en | en_US |
| dc.pages | 138 | en_US |
| dc.publisher | Plant Pathology Department, N. M. College of Agriculture Gujarat Agricultural University | en_US |
| dc.sub | Plant Pathology | en_US |
| dc.subject | null | en_US |
| dc.theme | M.Sc. Thesis | en_US |
| dc.these.type | M.Sc | en_US |
| dc.title | Interaction of Mungbean Yellow Vein Mosaic virus and Rhizobium Sp. in Mungbean [Vigna radiata (L.) | en_US |
| dc.type | Thesis | en_US |