Interaction of Mungbean Yellow Vein Mosaic virus and Rhizobium Sp. in Mungbean [Vigna radiata (L.)
Loading...
Date
2014-07
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
Plant Pathology Department, N. M. College of Agriculture Gujarat Agricultural University
Abstract
Yellow Mosaic Disease of the mungbean caused by a variant
MYMV-Vig: IN: NVS: Mg: 2012 in the South Gujarat.
Rhizobium
sp. is a symbiotic nitrogen fixing bacteria remain associated with the
legumes. The present research work was aimed to find the interaction
of Rhizobium sp. and MYMV for the exploiting the mechanism to
reduce the disease and improve the yield and productivity. Ten
different samples were collected to isolate the Rhizobium strain of
mungbean.
Through
the
isolation
and
initial
biochemical
characterization. three isolates (NAURh-l. NAURh·2 and NAURh-7)
out of the ten isolates were suspected to be the Rhizobium sp. They
•
I
showed
white,
round,
convex,
glistening,
mucoid
and
opaque
colonies on YEMA. These isolates showed negative reaction in
IMViC test, H 2 S test, starch hydrolysis test, caesin hydrolysis test,
nitrate reduction test,
Gelatin
hydrolysis test,
whereas
positiveAbstract
reaction in oxidase and urea hydrolysis test, All the three
isolates
,
look alike, therefore, only one isolate NAURh-2 was promoted for
the
further
research.
Among
the
different
antibiotic
tested,
Sparfloxacin and Tetracycline were placed in the categories of
ch loram phen ico I.
intermediate;
co-tri moxazo Ie,
levofloxacin,
ofloxacin, cefotaxime, gentamycin, tobramycin and streptocycline
were
susceptible
oxacillin,
•
and
•
Im'penem,
ciprofloxacin,
meropenem,
cafalaxin,
cephalothion ,
moxifloxacin,
amoxyclav,
penicillin, ampicillin, erythromycin, vanomycin, clindamycin and
sulphatriad were of resistant categories. Through the Biolog study
and subsequently manual analysis of the data and biological studies
it
was
concluded
that
the
isolate
(NAURh-2)
was
Rhizobium
iegllmil1osarllm . Cell length of the bacteria varied from 1.2 to 3.0
I'm, whereas cell width varied from 0.5 to 1.0 I'm . The cells from the
freshly prepared culture were Gram negative and found regular rod
shaped. Cells were either singly or arranged in aggregates. No spore
formation was observed even on the old and stressed cultures.
Bacteria produces capsule were motile with peritrichous flagella .
Nodulation in the crop could be seen at the 12 days onwards after the
inoculation. Maximum 28 nodules per plant were observed at 32 post
inoculation thereafter , it started decreasing in the present situation.
Detail steps of the nodulation were studied. Significantly higher
nodules were observed in the plants inoculated from the Rhizobium
ieguminosarllm
treatment,
seed
in
the
control
treatment
with
condition.
the
Among
Rhizobilllll
the
different
iegllmil10sarumAbslraCI
IOmllkg was found significantly superior. No effect
~f
the early
nodulation on the Rhizobium sp . nodulation and vice versa was
observed. If this would have been observed as per the few published
literature, this would have been exploited for the minimization of
losses caused by the MYMV and enhancement of productivity of the
high demanded protein source of vegetarian.
Description
Keywords
null
Citation
CHUDASAMA MITESH KHIMJIBHAI_64804