STUDIES ON CULTIVATION AND ANTIMICROBIAL PROPERTIES OF Pleurotus SPECIES UNDER SUBTROPICAL ZONE OF HIMACHAL PRADESH (H.P)
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Date
2019-10-22
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COLLEGE OF HORTICULTURE AND FORESTRY, DR Y S P UHF, NERI, HAMIRPUR
Abstract
The present investigations entitled “Studies on cultivation and antimicrobial properties of Pleurotus species under subtropical zone of Himachal Pradesh (H.P)” were undertaken to evaluate different locally available substrates and substrate supplementation for the production of P. florida, P. sajor-caju and P.ostreatus and to study the antimicrobial activities of these Pleurotus species against human pathogenic bacteria.Out of five different substrates tested, paddy straw proved to be the best in minimising time taken for spawn run (8.89 days) and producing maximum number of fruit bodies (52.52) while, wheat straw proved best in terms of maximum average yield (507.33 g/2Kg wet substrate) as well as biological efficiency (84.55 %). Among the three Pleurotus spp., P. florida performed best in terms of yield. Maximum cap diameter (66.59 mm) and stipe length of fruit bodies (23.56 mm) were observed in crop raised on wheat straw and Paddy straw, respectively. However, among three Pleurotus species, maximum cap diameter (57.42 mm) was observed in P. sajor-caju while, maximum stipe length (22.20 mm) was observed in P. florida. Out of eight supplements amended in wheat straw substrate, wheat bran supplemented at the rate of 7 per cent proved best in terms of minimum time taken for spawn run (7.00, 7.33 and 7.33 days, respectively) and maximum yield (554.00, 540.67 and 507 g/2Kg wet substrate, respectively) as well as biological efficiency (92.37, 90.11 and 84.50 %, respectively) for the cultivation of P. florida, P. sajor-caju and P. ostreatus which was statistically at par with rice bran at the same rate. Antimicrobial properties of different species of Pleurotus were tested by using the aqueous, hot water, ethanolic and methanolic extracts of mycelia, pileus and stipe against human pathogenic bacteria viz., Echerichia coli, Pseudomonas sp., Proteus sp., Bacillus sp. and Streptococcus sp. Among aqueous extracts, stipe extracts of all the three species were effective in inhibiting the growth of human pathogenic bacteria (9.33 mm), P. sajor-caju being the most effective in terms of inhibiting all the five test bacteria. However, hot water, ethanolic and methanolic extracts of pileus of three Pleurotus spp. were found to be most effective in terms of exhibiting maximum zone of inhibition (8.16, 6.64 and 7.23 mm, respectively). Among the three species, hot water extract of P. ostreatus (3.20 mm) and ethanolic as well as methanolic extracts of P. sajor-caju (2.09 and 4.02 mm, respectively) were most effective against the test bacteria.
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