INFLUENCE OF MICROBIAL CULTURES ON BIOLOGICAL QUALITY OF POLLUTED SOILS AND SPINACH YIELD
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Date
2014
Authors
UMA RAJASHEKHAR, ALAVALA
Journal Title
Journal ISSN
Volume Title
Publisher
ACHARYA N.G. RANGA AGRICULTURAL UNIVERSITY, RAJENDRANAGAR, HYDERABAD
Abstract
The experiment was conducted during 2012-13 at Department of Agricultural
Microbiology and Bioenergy, College of Agriculture, Rajendranagar, Professor
Jayashankar Telangana State Agricultural University, Hyderabad.
The treatments for poly bag experiment were fixed and twelve treatments each
treatment with three replications were designed. The cleaned poly bags were filled with 8
kg of soil and this soil was mixed with chemical fertilizers (0.14: 0.24: 0.37 g poly bag-1
NPK), farm yard manure (78.75 g poly bag-1) and Vesicular Arbuscular Mycorrhizae (100
to150 g of infected propagules poly bag-1) according to the treatments which were neatly
arranged in the net house.
The poly bag experiment was conducted following complete randomized block
design with 12 treatments and three replications. Treatments consisted of T1 SF Soil +
FYM @ 12 T/ha, T2: SF Soil + FYM + VAM + Pseudomonas, T3: SF Soil + RDF, T4: SF
Soil + RDF + FYM + VAM + Pseudomonas in polluted soil with supply of fresh water T5:
Soil + FYM+ Pseudomonas, T6: Soil + FYM + VAM + Pseudomonas, T7: Soil + RDF , T8:
Soil + RDF + FYM + VAM + Pseudomonas in unpolluted soil with supply of fresh water,
T9: Soil + FYM, T10: Soil + FYM + VAM + Pseudomonas, T11: Soil + RDF, T12: Soil +
RDF + FYM + VAM + Pseudomonas in unpolluted soil with supply of polluted water.
Microbial population and enzyme activity of enzymes urease, dehydrgenase, acid and
alkaline phosphatases were recorded at initial, 30 and 60 DAS. Available soil nitrogen,
phosphorus and potassium were recorded at initial and 60 DAS. Dry matter accumulation
i.e. fresh and dry weight (g plant-1) were recorded at 30 and 60 DAS. Microbial biomass
carbon and leaf area measured at 60 DAS. The Ni, Co, Cd in stem & leaf portion of spinach
plants analyzed by Atomic absorption spectrophotometer. The water samples were
collected and analysed for BOD and COD.
Effect of microbial cultures on microbial population at 30 and 60 DAS (107CFU
g-1 of soil in polluted and unpolluted soils of spinach beet showed best result for the
treatment T9: Soil + FYM and T12: Soil + RDF + FYM + VAM + Pseudomonas. Effect of
microbial cultures on enzymes urease, dehydrogenase, acid and alkaline phosphatases were recorded maximum enzyme activity in Treatment T8: Soil + RDF + FYM + VAM +
Pseudomonas in unpolluted soil with supply of fresh water than all other treatments.
Higher available nitrogen, phosphorus, potassium in the soil were observed in the
treatment receiving T3: SF Soil + RDF which was significantly higher than the treatments.
Highest leaf area and fresh and dry weight of leaf per plant were recorded in treatment T8:
Soil + RDF + FYM + VAM + Pseudomonas in unpolluted soil with supply of fresh water.
Heavy metal content in soil, leaf and shoot were highest for the treatments receiving SF
Soil + RDF + FYM + VAM + Psuedomonas in polluted and unpolluted soils.
Description
Keywords
INFLUENCE, MICROBIAL, CULTURES, BIOLOGICAL, QUALITY, POLLUTED, SOILS, SPINACH, YIELD