Sex Determination in African Love Birds (Agapornis Sp.) Using P2/P8 set of Primers by PCR

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Date
2017-05
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Indian Veterinary Journal
Abstract
Most of the bird species are not sexually dimorphic. In such cases, molecular methods are more efficient for their sex determination. The objective of this study was to test the feasibility and efficacy of feathers as a molecular method of sex determination in African love birds. From the freshly plucked down feathers, DNA was isolated and the amplification of the CHD gene was performed using P2/P8 set of primers. Sexing was successfully determined in 24 African love birds. P2/P8 primers proved to work well for sexing in African love birds. The males showed single bands and the females showed double bands.
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Veterinary Science, Wildlife Science
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