Rapid multiplex detection of food borne pathogens from ready to eat food products

Simple item page
dc.contributor.authorRathnapraba, S
dc.contributor.authorSaranya, S
dc.contributor.authorTANUVAS
dc.date.accessioned2020-03-04T09:56:37Z
dc.date.available2020-03-04T09:56:37Z
dc.date.issued2020-02
dc.descriptionTNV_20thMVC_PP_Feb-2020_OP12en_US
dc.description.abstractFood safety is increasingly becoming an important public health issue, as foodborne diseases present a widespread public health problem globally. The rapid and precise monitoring and detection of foodborne pathogens are some of the most effective ways to control and prevent foodborne iillness. In the present study, Antimicrobial peptide (AMP) coupled magnetic nanoparticles (MNPs) were prepared and utilized for entrapment of foodborne pathogens from food samples and coupled with multiplex PCR assay. Multiplex PCR primers were designed targeting the Fem A, Tuf and HL genes to detect the three bacterial pathogens such as Staphylococcus aureus, Bacillus cereus and Streptococcus spp. simultaneously. ATCC reference strains were used to standardize the multiplex PCR. A total of about 250 meat and milk products were screened for thepresence of targeted foodborne pathogens by the developed mPCR. The results indicated that the developed mPCR for rapid detection of foodborne pathogens had maximum of 89%, 97% and 93% of sensitivity, specificity and accuracy respectively. Hence, the novel antimicrobial peptide nanoparticle based multiplex PCR could be a rapid detection assay to assess food safety.en_US
dc.identifier.urihttp://krishikosh.egranth.ac.in/handle/1/5810144204
dc.keywords20th Indian Veterinary Congress, Annual Conference of IAAVR, TANUVASen_US
dc.language.isoenen_US
dc.pages1en_US
dc.subjectVeterinary Scienceen_US
dc.titleRapid multiplex detection of food borne pathogens from ready to eat food productsen_US
dc.title.alternativeNational Symposium on Veterinary Research Priorities in Translational Animal Health, Production and Food Safetyen_US
dc.typePresentationen_US
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
TNV_20thMVC_PP_Feb-2020_OP12.pdf
Size:
108.12 KB
Format:
Adobe Portable Document Format
Description:
TNV_20thMVC_PP_Feb-2020_OP12
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
Poster Presentations