EVALUATION OF A CANDIDATE GNP-APRV2 FOOTROT NANOVACCINE IN MICE
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Date
2017
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CSKHPKV, Palampur
Abstract
Footrot is one of the most important diseases of small ruminants worldwide. It is a chronic debilitating disease causing separation of hoof leading to severe lameness. Dichelobacter nodosus, a multi-serogroup gram negative anaerobic rod is principal causative agent of footrot. Disease causes heavy loss to sheep industries every year in terms of expenditure incurred in treatment programs, however treatment is of limited value so focus is shifted towards prophylactic immunisation. Currently available footrot vaccines provide only serogroup-specific immunity. Acidic serine protease (Aprv2) secreted by virulent D. nodosus can serve as a better vaccine candidate as it is conserved in all the 10 serogroups. Gold nanoparticles have been proved to possess great potential as antigen carrier and adjuvant, therefore in this study, a candidate gold nanoparticle-AprV2 vaccine was evaluated against footrot in mice. Recombinant AprV2 was expressed and purified followed by its adsorbtion over the surface of gold nanoparticles (18-20nm). Five groups consisting of 10 mice in each group were administered with three doses of GNP-AprV2 nanovaccine, GNP-AprV2 nanovaccine with MPLA, AprV2, GNP and PBS, respectively at a fortnight interval to evaluate the immune response by measuring IgG levels. GNP AprV2-nanovaccine induced higher IgG responses in comparison to AprV2 and MPLA adjuvanted GNP-AprV2 nanovaccine when measured at 45 days. However, the level of IgG were higher for MPLA at earlier time points (14 & 28 days).
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