Management of root knot nematode (Meloidogyne enterolobii) in tomato and guava using bio-intensive approaches
| dc.contributor.advisor | Sharma, Roopali | |
| dc.contributor.author | Shubham Kumar | |
| dc.date.accessioned | 2019-09-21T06:19:25Z | |
| dc.date.available | 2019-09-21T06:19:25Z | |
| dc.date.issued | 2019-08 | |
| dc.description.abstract | Meloidogyne enterolobii is a tropical or subtropical nematode and has a broad host range, including cultivated plants and weeds. For decades, the control of sedentary nematodes has relied heavily on chemical nematicides. In order to avoid excess use of nematicides, other alternates for ecofriendly management techniques like Biological control and Biofumigation can be explored. The present investigations were carried out to study bio-intensive management of Meloidogyne enterolobii causing root-knot in tomato and guava. The Identification of root knot nematode (Meloidogyne enterolobii) was done microscopically. The females of M. enterolobii showed characteristic pear-shape and pearly-white colour. The perineal pattern of adult females showed an oval shape dorsal arch which is usually high and round and weak lateral lines sometimes present with large phasmids. In vitro effect of Biocontrol Agents namely, Trichoderma asperellum (Th-14) and Pseudomonas fluorescens (Psf-173) and Biofumigants namely, Brassica juncea (mustard) variety Kranti and Raphanus sativus (raddish) variety Japanese white, was studied on the hatchability of eggs and mobility of second stage juvenile (J2) of M. enterolobii. Results revealed that, the rate of egg hatching and mobility of J2 decreases with the exposure of Biocontrol Agents and Biofumigants in different concentrations. Among all treatments, maximum reduction in egg hatching was recorded in Brassica juncea (67.00%) followed by Raphanus sativus (63.73%), Trichoderma asperellum (56.04 %) and Pseudomonas fluorescens (46.15%) after 96 hours of incubation period at 15 percent concentration over control (91.00% hatching of eggs). While, in case of juvenile mortality, Brassica juncea showed that maximum mortality of juveniles (92.60%) followed by Raphanus sativus (87.36%), Trichoderma asperellum (60.00%), Pseudomonas fluorescens (55.78%) over control (5% mortality of juveniles) at 15 concentration and incubation period of 96 hours. Scanning Electron Microscopy (SEM) was performed to study the mode of action of Trichoderma on the eggs of Meloidogyne enterolobii. Mycelial parasitization of eggs through Trichoderma showed complete coiling which finally resulted in the desiccation and killing of eggs. The management of root knot nematode (M. enterolobii) using biointensive approaches was studied under the glass house conditions in tomato and guava. The experiments were laid out using different treatments of Biocontrol agents (Trichoderma asperellum (Th-14), Pseudomonas flourescens (Psf-173), PBAT 3 (consortium of Th14 & Psf173) and Paecilomyces lilacinus (commercial) with the combination of Biofumigation (Brassica juncea & Raphanus sativus). Nematicide carbofuran was used as chemical check. All the treatments showed positive effect on the growth parameters of tomato and guava plants. In tomato, combination of BAT3+Biofumigation(M) resulted in maximum fresh root (7.38g) and shoot weight (36.07g) with minimum number of root galls (18.33galls per root system) as compared to the combination of other treatments. Whereas, in guava, maximum fresh root weight was found in the case of both treatments CF+Biofumigation(R) (2.57g) and Psf173+Biofumigation(R) (2.57g) which was statistically similar to PBAT3+Biofumigation(R) (2.51g). Minimum number of root galls (17.67galls per root system) was observed in PBAT3+Biofumigation(M). | en_US |
| dc.identifier.uri | http://krishikosh.egranth.ac.in/handle/1/5810128841 | |
| dc.keywords | insect pest management, Meloidogyne enterolobii, tomatoes, guavas | en_US |
| dc.language.iso | en | en_US |
| dc.pages | 103 | en_US |
| dc.publisher | G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand) | en_US |
| dc.research.problem | Tomatoes | en_US |
| dc.sub | Plant Pathology | en_US |
| dc.subject | null | en_US |
| dc.theme | Insect Pests | en_US |
| dc.these.type | M.Sc | en_US |
| dc.title | Management of root knot nematode (Meloidogyne enterolobii) in tomato and guava using bio-intensive approaches | en_US |
| dc.type | Thesis | en_US |