Comparative assessment of the adaptive capacity of goat to the heat stress based on genotype and phenotype

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dc.contributor.advisorMishra, Chinmoy
dc.contributor.authorVenkatesh, K.M.
dc.contributor.otherNayak, G.D.
dc.contributor.otherBehera, K.
dc.contributor.otherMishra, S.R.
dc.contributor.otherPradhan, S.K.
dc.date.accessioned2022-02-15T08:15:26Z
dc.date.available2022-02-15T08:15:26Z
dc.date.issued2021
dc.descriptionTH - 6648en_US
dc.description.abstractMelanogenesis is primarily mediated by the melanocortin receptor type 1 gene. The present study was carried out to study the effect of polymorphism on animal coat colour and its association with heat stress indicators such as rectal temperature, skin temperature, respiration rate, heart rate, and cortisol level in goats under study. The genotyping (Dideoxy Sanger sequencing) result of the present study revealed three SNPs each in the Ganjam and Keonjhar goat populations and combinedly made four SNPs at 183rd (Y), 332nd (S), 748th (K) and 801st (S) positions. A novel SNP was observed at 332th (S) position in the Keonjhar goat population. Two amino acid changes (F250V and C267W) were observed due to the missense variation of nucleotides. Eight haplotypes were constructed from the unphased nucleotide sequences using the algorithm. The association between coat colour and the recorded traits revealed that white animals with the "CCTC" haplotype had significantly (P<0.05) less rectal temperature, skin temperature, respiration rate, and cortisol values as compared to black and brown animals. The principal component analysis revealed that rectal temperature, skin temperature, respiration rate, and cortisol values were related to each other and contributed more to the variance components. The different gene flow and genetic differentiation parameters for the total population, Ganjam goat and Keonjhar goat populations were estimated. The histomorphology study of the skin of different coat-coloured goats supported the results of the present study that white animals were less heat-stressed as compared to black and brown goats. The presence of calcium ion, which plays a critical role in the ligand and receptor binding, doesn’t alter the confirmation and the complexes formed between the MC1R protein and α-MSH ligand during the docking study. In conclusion, according to the findings of the present study, the white coat colour of the animal may be the reason for the white animals' lower heat stress levels as compared to the black and brown coat colours of the animals. The haplotype CCTC, which was linked to the white coat colour of the animals, suggests an association between the haplotype and white animals' heat tolerance.en_US
dc.identifier.urihttps://krishikosh.egranth.ac.in/handle/1/5810182316
dc.keywordsColour, Gene, Goats, Heat stress, MC1Ren_US
dc.language.isoEnglishen_US
dc.pages88pen_US
dc.publisherDepartment of Animal Breeding and Genetics, OUAT, Bhubaneswaren_US
dc.subAnimal Genetics and Breedingen_US
dc.themeColour, Gene, Goats, Heat stress, MC1Ren_US
dc.these.typeM.V.Sc.en_US
dc.titleComparative assessment of the adaptive capacity of goat to the heat stress based on genotype and phenotypeen_US
dc.typeThesisen_US
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