Insilico characterisation and epitope profiling of differentially expressed plasma membrane proteins of ‘x’ and ‘y’ chromosome bearing bovine sperm
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Date
2021-09
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G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand)
Abstract
The specific and differentially expressed plasma membrane proteins of bovine sex sorted semen were selected after reviewing numerous research publications on proteomic profile of sexed semen of bovines. The insilico characters such as physicochemical properties, primary, secondary, tertiary structures, membrane topology, sub-cellular localisation, domain analysis, protein interactions and epitope profiling, both linear and discontinuous peptides was done for all the proteins by using various bioinformatics tools and software. For X specific protein CLRN3, 6 and 4 and for Y chromosome specific protein, SCAMP1, 6 and 8 linear and discontinuous epitopes were predicted, respectively. The proteins were localized on Plasma membrane like SPACA1, Leucine-rich repeat and fibronectin type III domain containing 2, CLRN3, SCAMP1 and Uncharacterised Protein may act as antigenic protein to be used for further production of sex- specific antibodies and separation of X and Y sperm. The results of insilico characterisation and epitope prediction of various selected X and Y bovine sperm plasma membrane specific and differentially expressed proteins would be helpful in selection of some efficient antigenic proteins to be used for further X and Y sperm specific antibody production or synthesis of antibodies artificially.