Biochemical and molecular attributes of the diverse genetic pool of Brassica juncea var. rugosa (pahari rai) for identification of elite germplasm having potent bioactivity

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dc.contributor.advisorPunetha, Himanshu
dc.contributor.authorAdhikari, Ankur
dc.date.accessioned2023-02-11T09:11:41Z
dc.date.available2023-02-11T09:11:41Z
dc.date.issued2022-09
dc.description.abstractBrassica juncea var. rugosa (pahari rai), a broad leafy cruciferous vegetable is an essential and easy source of nutrition with numerous health-promoting factors. Eighteen different germplasms of Pahari rai and two checks (Pusa saag and Kranti) have been cultivated during seasons 2019-20 and 2020-21. Germplasm had totally different selected agro-morphological traits. PCA analysis depicted that PC1 (35.25%), PC2 (18.71%) and PC3 (17.09%) contributed to the morphological variability among the germplasms for the traits under study. Tocher method divided all germplasms into four different clusters as per morphological characters. A biplot between PC1 & PC2 showed, germplasm IC-399826 is within the ace of origin were more stable and less diverse, whereas IC-597821, IC- 298019 and IC-276011 are away from the origin, which were less stable and more diverse. Genetic diversity and population structure of cultivated germplasms using 100 SSR primers subjected to Darwin 5 software. A total of 74% SSRs resulted in polymorphic amplicons. UNJ-based dendrogram grouped all the 18 germplasm along with two checks into two distinct clusters. PCA of genetic diversity showed that the first and second principal components accounted for 29.25% and 14.7% of the total variation. Phytochemical and antioxidant activity of leaf extracts showed the highest phenolic (6.45±0.045 mg GAE g-1), flavonoid (6.32±0.06 mg QE g-1), ODP (4.66 ± 0.52 mg CE g-1) and tannin (3.46 ± 0.02 mg tannic acid g-1) content were observed in the EEC-25 leaf methanolic extract. The highest protein content in EEC-25 (400.97±16.5 μg QE g-1) followed by IC-524259 (380.09±17.22 μg QE g-1) and IC-350800 (379.23±18.26 μg QE g-1). The foremost concentration of carbohydrates was observed in germplasms IC-363758 (31.47±0.31 mg glucose g-1), IC-410471 (31.12±0.18 mg glucose g-1) and EEC-25 (26.45±0.19 mg glucose g-1). In-vitro antioxidant potential viz., total antioxidant content was observed highest in EEC-25 methanolic leaf extract (41.91±0.28 mg AAE g-1), FRAP activity in EEC-25 (5.91±0.68 mg AAE g-1), maximum DPPH free radical scavenging activity in IC- 597933 (IC50=21.45±3.4 μg mL-1), ABTS free radical scavenging activity in IC-524259 (94.17±0.48 μg mL-1), superoxide radical scavenging activity of methanolic extract of PR-15 (IC50=124.37 μg mL-1), highest metal ion chelating activity of EEC-25 methanolic extract (IC50=52.22 μg mL-1), maximum reducing power activity in EEC-25 and IC-399839, all being significantly different (p≤0.05) from each other. Positive correlations have been observed among the total antioxidant, total phenolic, total flavonoid content, ODP, DPPH and FRAP. Pahari rai was observed to be a rich source of MUFA and PUFA. An essential metric for assessing the nutritional value of various edible oils is the ω6:ω3 ratio was observed to be maximum in IC-350800 (2.53), IC-338535 (2.34) and IC-410471 (2.19). Micronutrient analysis Pahari rai leaf observed to be the rich source of essential minerals such as Mn, Fe, Zn, Ca, Cu and Mg. The anti-inflammatory activity among the elite accessions the IC-350800 (IC50 =25.19±1.06 μg mL-1) was observed to be the maximum. No zone of inhibition was evident against selected human pathogenic bacteria using methanolic leaf extracts of elite accessions of Pahari rai. Invitro cytotoxicity in MDBK and Vero against the selected methanolic leaf extracts (IC-524259, EEC- 25, IC-597933, IC-350800 and Pusa saag) was non-significant. Results clearly revealed the presence of significant amounts of phytochemicals, antioxidant activities and in vitro anti-inflammatory activities in leaves of Pahari rai. The multiutility of this leafy vegetables against numerous pathological ailments was attributed to pharmacological activities of phytochemicals and for the development of functional food for human consumption.en_US
dc.identifier.urihttps://krishikosh.egranth.ac.in/handle/1/5810193659
dc.keywordsBrassica juncea, genetic studiesen_US
dc.language.isoEnglishen_US
dc.pages144 p.en_US
dc.publisherG. B. Pant University of Agriculture and Technology, Pantnagaren_US
dc.research.problemGenetic studiesen_US
dc.subBiochemistryen_US
dc.themeBrassica junceaen_US
dc.these.typePh.Den_US
dc.titleBiochemical and molecular attributes of the diverse genetic pool of Brassica juncea var. rugosa (pahari rai) for identification of elite germplasm having potent bioactivityen_US
dc.typeThesisen_US
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