Principle component analysis of genetic structure in Kilakarsal and Vembur sheep breeds by using single nucleotide polymorphic (SNP) markers within TLR genes

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dc.contributor.authorSelvam, R.
dc.contributor.authorMurali, N.
dc.contributor.authorKumar, V. Ramesh Saravana
dc.contributor.authorPonnudurai, G.
dc.contributor.authorTANUVAS
dc.contributor.authorThiruvenkadan, AK
dc.date.accessioned2018-08-30T12:17:17Z
dc.date.available2018-08-30T12:17:17Z
dc.date.issued2017
dc.description.abstractThe genetic structure among the Kilakarsal and Vembur sheep were studied by principal components analysis. A total of 25 SNP (each five SNP in TLR3, TLR5, TLR6, TLR9 and TLR10) were utilized for genotyping of Kilakarsal and Vembur sheep by using competitive allele specific PCR assay. The allele frequencies and allele sharing genetic distances (based on identical by state) among pairs of individuals within and across different sheep breeds was estimated by using PEAS program. Pair-wise inter individual allele sharing distance among both the sheep breeds were utilized to perform principal components analysis using SPSS. Among 25 SNPs analysed, 22 were found to be polymorphic in both Kilakarsal and Vembur sheep. The pairwise allele sharing distances indicated that certain individuals across populations were more related than individuals within populations. The top three principal components explained respectively 45.1, 20.2 and 11.9 % of the total variation and together explained 77.2 % of the variation in the dataset. The top eight principal components had eigen values greater than one and cumulatively explained 97.48 % of the total variation in the dataset. Principal components scattergam did not reveal the presence of cryptic genetic structure among the two sheep breeds under studyen_US
dc.identifier.urihttp://krishikosh.egranth.ac.in/handle/1/5810070234
dc.keywordsVeterinary Science, Livestock Production and Management, allele, KASP, PCR, PEASen_US
dc.language.isoen_USen_US
dc.pages415-420en_US
dc.subjectVeterinary Scienceen_US
dc.subjectLivestock Production and Managementen_US
dc.titlePrinciple component analysis of genetic structure in Kilakarsal and Vembur sheep breeds by using single nucleotide polymorphic (SNP) markers within TLR genesen_US
dc.typeArticleen_US
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