EVALUATION OF GENETIC RELATEDNESS AMONG TEMPERATE POME FRUIT CROPS OF FAMILY ROSACEAE USING PCR BASED MOLECULAR MARKERS
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Date
2014
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UHF,NAUNI
Abstract
ABSTRACT
The family Rosaceaeis third most economically important plant family that include
temperate pome fruit crops having a wide genetic diversity. For a rationale use of the genetic
resources in breeding programs it is necessary to understand relationship within and among
related crop varieties and to assess their transferability to the species with little genomic
information making them significant for genetic research. Thus, in the present study genetic
relatedness and SSRs transferability among fifty genotypes of temperate pome fruit crops
were tested using (RAPD, ISSR and SSR) molecular marker analysis. The level of
polymorphism across genotypes was 97.29% and 97.45% by RAPD and ISSR markers,
respectively. The dendrogram derived from RAPD and ISSR analysis grouped temperate
pome fruit crops into two major cluster comprising of 40 (cluster A) and 10 (cluster B)
genotypes which branched at similarity value of 0.31 and 0.33, respectively. Estimates of
pooled similarity coefficient ranged from 0.30 to 0.94. It was concluded from the pooled
analysis of both the molecular marker that 50 genotypes of temperate pome fruit crops were
grouped in two clusters A and B comprised 45 and 5 genotypes, respectively which branched
at similarity value of 0.30 with the maximum similarity of 94% was found between EC-02520
and EC-024530 quince genotypes. Further, out of 13 and 15 screened apple and pear genomic
SSR, 10 (76.92%) and 11 (73.33%) produced amplification in 20 apple and pear genotypes,
respectively. Highest transferability of apple and pear SSR, 61.53% and 73.33% was observed
in closely related quince and apple genotypes, respectively. This high level of polymorphism
and good transferability of apple and pear SSRs to other temperate pome fruit crops indicated
their promise for application to future molecular screening, map construction, and
comparative genomic studies, etc.
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