Evaluation of antioxidant property of Selenium biofortified paddy straw mushroom, Volvariella volvacea

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dc.contributor.advisorSuman Kumari
dc.contributor.authorJashanpreet Kaur
dc.date.accessioned2021-07-28T04:16:19Z
dc.date.available2021-07-28T04:16:19Z
dc.date.issued2021
dc.description.abstractSelenium is one of the essential mineral nutrients, required by human body as involved in antioxidant and defense reactions. Biofortification of mushrooms using naturally enriched agricultural substrates can provide a productive use of straw in producing enriched mushrooms with high biological efficiency, nutritional as well as nutraceutical value. In this context, the present study focused on evaluation of the effect of Se supplementation on two strains of V. volvacea (VV3 and VV6), under in vitro as well as in vivo conditions. In vitro studies revealed that Selenium (Se) supplementation of 10 and 15ppm maximally enhanced the radial growth rate of VV3 and VV6 respectively, among all the treatments including control. Mycelial proliferation in liquid medium was recorded highest in case of 15 (12.09±0.42 g/l) and 10 ppm (12.30±0.60 g/l) in case of VV3 and VV6. Scanning Electron Microscopic observations for the effect of Se on hyphal growth disclosed sparse, non uniform damaged hyphae with increasing concentration of Se at 20 ppm, compared to regular, dense healthy hyphae in control in both the strains. Mushroom cultivation on Se-rich paddy straw collected from seleniferous soil showed significant increase in yield (573±60 and 604±87 g/bed) in strain VV3 and VV6, compared to non Se paddy straw grown mushrooms i.e., 432.24 ±64.64 and 543.34±32.24 g/bed average yield respectively. The positive influence of Se biofortification was also observed on antioxidative activity of V. volvacea both under in vitro and in vivo on protein content, total phenols, flavonoids, Peroxidase (PO) and free radical scavenging activity. The antioxidants were found to increase with the increase in Se supplementation, however when the concentration exceeded 15ppm, the levels were reduced due to toxic effects of Se under axenic conditions. Biosorption studies for Se using ICPMS revealed highest Se in 20pmm supplementation i.e. 576.01±12.98 and 623.27±10.74 µg/g dw in VV3 and VV6 under in vitro. In case of fruiting bodies, highest total Se was observed in VV6 (57.81±4.16) than VV3 (53.43±4.78) that was significant over the respective controls with 4.13±0.28 and 2.64±0.41 µg/g dw. The functional group diversity analysis using Fourier Transform Infra-red Spectroscopy (FT-IR) confirmed changes in structural and conformational changes in biomolecules in Se enriched mycelia and fruiting body. The present study concluded that biofortification of Selenium, improved the yield as well as antioxidative property of V. volvacea compared to control and biofortification of essential nutrients using rich agricultural residues can be an effective and ecofriendly alternate for paddy straw disposal.en_US
dc.identifier.citationJashanpreet Kaur (2021). Evaluation of antioxidant property of Selenium biofortified paddy straw mushroom, Volvariella volvacea (Unpublished M.Sc. thesis). Punjab Agricultural University, Ludhiana, Punjab, India.en_US
dc.identifier.urihttps://krishikosh.egranth.ac.in/handle/1/5810170945
dc.keywordsAntioxidants, Biofortification, Selenium, Volvariella volvacea.en_US
dc.language.isoEnglishen_US
dc.pages84en_US
dc.publisherPunjab Agricultural University, Ludhianaen_US
dc.research.problemEvaluation of antioxidant property of Selenium biofortified paddy straw mushroom, Volvariella volvaceaen_US
dc.subMicrobiologyen_US
dc.themeEvaluation of antioxidant property of Selenium biofortified paddy straw mushroom, Volvariella volvaceaen_US
dc.these.typeM.Scen_US
dc.titleEvaluation of antioxidant property of Selenium biofortified paddy straw mushroom, Volvariella volvaceaen_US
dc.typeThesisen_US
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