Evaluation of antioxidant property of Selenium biofortified paddy straw mushroom, Volvariella volvacea
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Date
2021
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Punjab Agricultural University, Ludhiana
Abstract
Selenium is one of the essential mineral nutrients, required by human body as involved in
antioxidant and defense reactions. Biofortification of mushrooms using naturally enriched
agricultural substrates can provide a productive use of straw in producing enriched mushrooms
with high biological efficiency, nutritional as well as nutraceutical value. In this context, the
present study focused on evaluation of the effect of Se supplementation on two strains of V.
volvacea (VV3 and VV6), under in vitro as well as in vivo conditions. In vitro studies revealed
that Selenium (Se) supplementation of 10 and 15ppm maximally enhanced the radial growth rate
of VV3 and VV6 respectively, among all the treatments including control. Mycelial proliferation
in liquid medium was recorded highest in case of 15 (12.09±0.42 g/l) and 10 ppm (12.30±0.60 g/l)
in case of VV3 and VV6. Scanning Electron Microscopic observations for the effect of Se on
hyphal growth disclosed sparse, non uniform damaged hyphae with increasing concentration of Se
at 20 ppm, compared to regular, dense healthy hyphae in control in both the strains. Mushroom
cultivation on Se-rich paddy straw collected from seleniferous soil showed significant increase in
yield (573±60 and 604±87 g/bed) in strain VV3 and VV6, compared to non Se paddy straw grown
mushrooms i.e., 432.24 ±64.64 and 543.34±32.24 g/bed average yield respectively. The positive
influence of Se biofortification was also observed on antioxidative activity of V. volvacea both
under in vitro and in vivo on protein content, total phenols, flavonoids, Peroxidase (PO) and free
radical scavenging activity. The antioxidants were found to increase with the increase in Se
supplementation, however when the concentration exceeded 15ppm, the levels were reduced due
to toxic effects of Se under axenic conditions. Biosorption studies for Se using ICPMS revealed
highest Se in 20pmm supplementation i.e. 576.01±12.98 and 623.27±10.74 µg/g dw in VV3 and
VV6 under in vitro. In case of fruiting bodies, highest total Se was observed in VV6 (57.81±4.16)
than VV3 (53.43±4.78) that was significant over the respective controls with 4.13±0.28 and
2.64±0.41 µg/g dw. The functional group diversity analysis using Fourier Transform Infra-red
Spectroscopy (FT-IR) confirmed changes in structural and conformational changes in
biomolecules in Se enriched mycelia and fruiting body. The present study concluded that
biofortification of Selenium, improved the yield as well as antioxidative property of V. volvacea
compared to control and biofortification of essential nutrients using rich agricultural residues can
be an effective and ecofriendly alternate for paddy straw disposal.
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Citation
Jashanpreet Kaur (2021). Evaluation of antioxidant property of Selenium biofortified paddy straw mushroom, Volvariella volvacea (Unpublished M.Sc. thesis). Punjab Agricultural University, Ludhiana, Punjab, India.