INTERACTION OF MUNGBEAN YELLOW VEIN MOSAIC VIRUS AND RHIZOBIUM SP. IN MUNGBEAN [Vigna radiata (L.) Wilczek]

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dc.contributor.advisorMahatma, Lalit
dc.contributor.authorChudasama, Mitesh K.
dc.date.accessioned2017-02-22T06:54:05Z
dc.date.available2017-02-22T06:54:05Z
dc.date.issued2014-07
dc.description.abstractYellow Mosaic Disease of the mungbean caused by a variant MYMV-Vig: IN: NVS: Mg: 2012 in the South Gujarat. Rhizobiu m sp. is a symbiotic nitrogen fixing bacteria remain associated wit h the legumes. The present research work was aimed to find the interaction of Rhizobium sp. and MYMV for the exploiting the mechanism to reduce the disease and improve the yield and productivity. Ten different sa mples were collected to isolate the Rhizobium strain of mungbean. Through the isolation and initia l bioche mical characterization, three isolates (NAURh-1, NAURh-2 and NAURh-7) out of the ten isolates were suspected to be the Rhizobium sp. They showed white, round, convex, glistening, mucoid and opaque colonies on YEMA. These isolates showe d negative reaction in IMViC test, H 2 S test, starch hydrolysis test, caesin hydrolysis test, nitrate reduction test, Gelatin hydrolysis test, whereas positive reaction in oxidase and urea hydrolysis test, All the three isolates look alike, therefore, only one isolate NAURh-2 was pro moted for the further research. Among the different antibiotic tested, Sparfloxacin and Tetracycline were placed in the categories of intermediate; chloramphenicol, co- trimoxazole, levofloxacin, ofloxacin, cefotaxime, gentamycin, tobramycin and streptocycline were susceptible and ciprofloxacin, cafalaxin, cephalothion, oxacillin, imipene m, meropenem, moxifloxacin, a moxyclav, penicillin, a mpicillin, erythro mycin, vano mycin, clindamycin and sulphatriad were of resistant categories. Through the Biolog study and subsequently manua l analysis of the data and biological studies it was concluded that the isolate (NAURh-2) was Rhizobium leguminosarum. Cell lengt h of the bacteria varied from 1.2 to 3.0 μm, whereas cell widt h varied from 0.5 to 1.0 μm. The cells from the freshly prepared culture were Gram negative and found regular rod shaped. Cells were either singly or arranged in aggregates. No spore formatio n was observed even on the old and stressed cultures. Bacteria produces capsule were motile with peritrichous flagella. Nodulation in the crop could be seen at the 12 days onwards after the inoculation. Maximum 28 nodules per plant were observed a t 32 post inoculation thereafter, it started decreasing in the present situation. Detail Significantly steps higher of nodules the were nodulation observed were in the studied. plants inoculated from the Rhizobium leguminosarum in the contro l condition. Among the different treatment, seed treatment with the Rhizobium leguminosarum 10ml/kg was found significantly superior. No effect of the early nodulation on the Rhizobium sp. nodulation and vice versa was observed. If this would have bee n observed as per the few published literature, this would have bee n exploited for the minimization of losses caused by the MYMV and enhance ment of productivity of the high de manded protein source of vegetarian.en_US
dc.identifier.urihttp://krishikosh.egranth.ac.in/handle/1/5810002113
dc.language.isoenen_US
dc.publisherPLANT PATHOLOGY DEPT., N.M.COLLEGE OF AGRICULTURE, NAVSARI AGRICULTURAL UNIVERSITY, NAVSARIen_US
dc.subPlant Pathologyen_US
dc.subjectnullen_US
dc.themeThesisen_US
dc.these.typeM.Scen_US
dc.titleINTERACTION OF MUNGBEAN YELLOW VEIN MOSAIC VIRUS AND RHIZOBIUM SP. IN MUNGBEAN [Vigna radiata (L.) Wilczek]en_US
dc.typeThesisen_US
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