INTERACTION OF MUNGBEAN YELLOW VEIN MOSAIC VIRUS AND RHIZOBIUM SP. IN MUNGBEAN [Vigna radiata (L.) Wilczek]
Loading...
Date
2014-07
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
PLANT PATHOLOGY DEPT., N.M.COLLEGE OF AGRICULTURE, NAVSARI AGRICULTURAL UNIVERSITY, NAVSARI
Abstract
Yellow Mosaic Disease of the mungbean caused by a variant
MYMV-Vig: IN: NVS: Mg: 2012 in the South Gujarat. Rhizobiu m
sp. is a symbiotic nitrogen fixing bacteria remain associated wit h
the legumes. The present research work was aimed to find the
interaction of Rhizobium sp. and MYMV for the exploiting the
mechanism to reduce the disease and improve the yield and
productivity. Ten different sa mples were collected to isolate the
Rhizobium strain of mungbean. Through the isolation and initia l
bioche mical characterization, three isolates (NAURh-1, NAURh-2
and NAURh-7) out of the ten isolates were suspected to be the
Rhizobium sp. They showed white, round, convex, glistening,
mucoid and opaque colonies on YEMA. These isolates showe d
negative reaction in IMViC test, H 2 S test, starch hydrolysis test,
caesin hydrolysis test, nitrate reduction test, Gelatin hydrolysis
test, whereas positive reaction in oxidase and urea hydrolysis test,
All the three isolates look alike, therefore, only one isolate
NAURh-2 was pro moted for the further research. Among the
different antibiotic tested, Sparfloxacin and Tetracycline were
placed in the categories of intermediate; chloramphenicol, co-
trimoxazole, levofloxacin, ofloxacin, cefotaxime,
gentamycin,
tobramycin and streptocycline were susceptible and ciprofloxacin,
cafalaxin,
cephalothion,
oxacillin,
imipene m,
meropenem,
moxifloxacin, a moxyclav, penicillin, a mpicillin, erythro mycin,
vano mycin,
clindamycin
and
sulphatriad
were
of
resistant
categories. Through the Biolog study and subsequently manua l
analysis of the data and biological studies it was concluded that
the isolate (NAURh-2) was Rhizobium leguminosarum. Cell lengt h
of the bacteria varied from 1.2 to 3.0 μm, whereas cell widt h
varied from 0.5 to 1.0 μm. The cells from the freshly prepared
culture were Gram negative and found regular rod shaped. Cells
were either singly or arranged in aggregates. No spore formatio n
was observed even on the old and stressed cultures. Bacteria
produces
capsule
were
motile
with
peritrichous
flagella.
Nodulation in the crop could be seen at the 12 days onwards after
the inoculation. Maximum 28 nodules per plant were observed a t
32 post inoculation thereafter, it started decreasing in the present
situation.
Detail
Significantly
steps
higher
of
nodules
the
were
nodulation
observed
were
in
the
studied.
plants
inoculated from the Rhizobium leguminosarum in the contro l
condition. Among the different treatment, seed treatment with the
Rhizobium
leguminosarum
10ml/kg
was
found
significantly
superior. No effect of the early nodulation on the Rhizobium sp.
nodulation and vice versa was observed. If this would have bee n
observed as per the few published literature, this would have bee n
exploited for the minimization of losses caused by the MYMV and
enhance ment of productivity of the high de manded protein source
of vegetarian.
Description
Keywords
null