Comparison of recombinant and whole cell leptospiral antigen against different cattle serovars of Ieptospira
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Date
2015-07
Journal Title
Journal ISSN
Volume Title
Publisher
Indian J. of Animal Sciences
Abstract
The outer membrane protein, viz. OmpL1 of Leptospira interrogans Icterohaemorrhagiae was cloned into pETl5b vector. The recombinant colonies were expressed in E. coli BL21 DE3 cells. The expressed proteins were checked in SDS—PAGE and western blot analysis. Similarly. the whole cell antigen was prepared using Leptospira interrogans Icterohaemorrhagiae culture.
Description
TNV_IJAS_85(7)_Jul2015_705-708
Keywords
Veterinary Science, Veterinary Physiology