ASSESSMENT OF GENETIC DIVERSITY OF Viola serpens Wall. IN HIMACHAL PRADESH USING MOLECULAR MARKERS
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Date
2010
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Abstract
ABSTRACT
Viola serpens Wall. commonly known as “Banakasha” belongs to family Violaceae. It is
one of the most useful medicinal plants and used as antipyretic, demulcent, diaphoretic and
diuretic drug. It is useful in asthma, bleeding piles, cancer of throat, constipation, cough, fever,
skin diseases and headache. In the present investigation an attempt has been made to assess
the genetic diversity of Viola serpens Wall. using morphological and isozymic markers. Ten
qualitative and seven quantitative characters have been studied but only quantitative
morphological descriptors showed significant variation. The isozyme analysis was conducted for
nine enzyme system and all of them showed significant activity producing typical banding
patterns. The eight isozymic bands viz. PER Ia, PER Ib, AAT Ia, EST I, CAT Ia, MDH Ia, ADH Ib
and PPH Ia successfully demarcated the six sites of Mandi, Hamirpur and Bilaspur districts from
four sites of Solan and Sirmaur districts. The two enzymes systems i.e. Alkaline phosphatase
and Acid phosphatase were found to be monomorphic in nature. According to dendrogram
constructed using UPGMA and NTSYS-pc software version 2.0 the thirty genotypes of ten sites
of five districts were bifurcated in to two major clusters the upper cluster consisted of the sites of
Mandi, Hamirpur and Bilaspur districts and lower cluster consisted of the sites of Solan and
Sirmaur districts. These clusters were further divided into sub-cluster and sub-sub-clusters. Allele
frequency in present study ranged from 0.5-1.0. Polymorphism in the selected thirty genotypes of
Viola serpens was found to be 26.67 per cent.