INDUCTION OF SOMATIC EMBRYOGENIC ACTIVITY IN POMEGRANATE (Punica granatum L.) CULTURES OF MATURE ORIGIN
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Date
2009-07-15
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University of Agricultural Sciences, Bangalore
Abstract
A study on inducing som atic embryogenic activity in pom egranate
was carried ou t by using explants of mature origin viz., petal, le a f and
anthers/stamens and m olecular profiling o f the embryogenic callus
issued from these explants w as carried out by using RAPD m arkers with
an objective o f developing standardized protocol for regeneration of
pomegranate, to develop cultivars resistant to bacterial blight pathogen
(Xanthomonas axonopodis pv. Punicae). U sing conventional breeding
procedures, it is difficult to develop resistant cultivars due to time and
space constraint. Hence, somatic embryogenesis offers viable protocols
since it produces both root and shoot meristems necessaiy for complete
plant growth. For inducing somatic embryos, explants of field origin were
collected. Initiation of growth and maintenance o f explants w as done on
MS and W PM semi-solid media. Callus initiation was seen after 3 and 8
weeks o f inoculation from petal and leaf explants respectively and
somatic em bryos were obtained. Response o f anthers/stamens w as seen
positively w hich produced embryogenic callus. Shoot apex form ation was
seen from petal and leaf derived embryogenic callus 22 w eeks of
inoculation. Callus issued from petal and anther was subjected for RAPD
marker analysis. 12 different primers OPG -03, O PG -05, OPG -08, OPG -
13, OPG -16 OPG -18, OPI-01, OPI -03, OPI -10, OPI -12, OPI -14 &
OPI -18 screened revealed clear amplification between petal derived and
anther derived callus from which it was strongly believed that the anther
derived callus is o f sporophytic origin.
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