IN VITRO PROPAGATION AND SECONDARY METABOLITE PRODUCTION IN Podophyllum hexandrum ROYLE (INDIAN MAYAPPLE)

Simple item page
dc.contributor.advisorTHAKUR, MANISHA
dc.contributor.authorSHARMA, NEHA
dc.date.accessioned2021-12-23T06:07:52Z
dc.date.available2021-12-23T06:07:52Z
dc.date.issued2021-12
dc.description.abstractABSTRACT The present study reports an optimized protocol for high frequency in vitro propagation through seeds and rhizome buds, molecular analysis and podophyllotoxin production in Podophyllum hexandrum. Maximum percent uncontamination of seeds (89%) was achieved on treatment with 0.1% HgCl2 for 3 minutes and highest percent surviving rhizome buds (82.62%) were obtained after surface sterilization with 0.1% HgCl2+1.0% NaOCl for 4 minutes with maximum in vitro establishment (95.97%) on MS medium fortified with 0.2mg/l BA and 0.1mg/l GA3. Maximum in vitro establishment through seeds (89.00%) and buds (90%) was achieved during spring, followed by 68 and 79% in winter season. The proliferated shoots from seeds showed highest multiplication (1:11) on MS medium fortified with 2.0mg/l BA + 0.2mg/l NAA+ 0.7mg/l CaCl2. Addition of CaCl2 (0.7mg/l) into the medium was done to prevent shoot chlorosis. For shoot regenerating from rhizome buds highest multiplication rate of 1:5 was achieved on MS medium fortified with 2.0mg/l BA +0.2mg/l NAA. Molecular analysis was done using SCoT and CBDP markers. SCoT markers showed 71% polymorphism in samples from different altitudes in comparison to 50.90% polymorphism depicted by CBDP markers. However, both the makers showed 100% monomorphism among mother plants and their tissue culture raised progeny. In vivo leaves and petioles as well as in vitro roots and leaves were used as explants for callus induction. Highest callus induction was observed under dark incubation in in vivo leaves (81.07%), petioles (87.19%), in vitro roots (90.67%) and leaves (89.20%).HPLC analysis revealed maximum (0.267%) production of podophyllotoxin from callus initiated from in vitro roots procured from experimental material of district Kinnaur followed by 0.258% from in vitro root callus of Lahaul – Spiti. For enhancing podophyllotoxin yield callus induced from in vitro roots of P. hexandrum plants of district Kullu were subjected to elicitation by incorporating different concentrations (0.5-1.5 mM) of elicitors (methyl jasmonate and chitin) in production medium. Highest podophyllotoxin content (0.33%) was observed after elicitation with 1mM methyl jasmonate followed by its higher concentration of 1.5 mM (0.31%).en_US
dc.identifier.urihttps://krishikosh.egranth.ac.in/handle/1/5810179753
dc.keywordsvitro propagation,seeds ,rhizome buds, molecular analysis , podophyllotoxin production, Podophyllum hexandrumen_US
dc.language.isoEnglishen_US
dc.pages62+ven_US
dc.publisherUHF,NAUNIen_US
dc.subBiotechnologyen_US
dc.themevitro propagation,seeds ,rhizome buds, molecular analysis , podophyllotoxin production, Podophyllum hexandrumen_US
dc.these.typePh.Den_US
dc.titleIN VITRO PROPAGATION AND SECONDARY METABOLITE PRODUCTION IN Podophyllum hexandrum ROYLE (INDIAN MAYAPPLE)en_US
dc.typeThesisen_US
Files
Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
PDF Ph.D. Thesis Neha Sharma (BTC).pdf
Size:
13.78 MB
Format:
Adobe Portable Document Format
Description:
BIOTECHNOLOGY
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
Theses