DETECTION AND CHARACTERIZATION OF IMPORTANT VIRUSES INFECTING BANANA IN SOUTHERN KARNATAKA
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Date
2023-03-30
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University of Agricultural Sciences, Bangalore
Abstract
Banana is one of the most popular and widely consumed fruits in the world. Banana is affected by many viral diseases. In the present investigation, detection and characterization of important viruses infecting banana in Southern Karnataka was carried out. The roving survey found that banana bunch top virus (BBTV) and banana streak virus (BSV) infection on banana in Chikkaballapura and Bengaluru rural districts. Under field conditions, infected banana plants exhibited typical symptoms of BBTV and BSV. The presence of these two viruses was confirmed by PCR, and multiplex PCR. Sequence and phylogenetic analysis revealed that nanovirus isolates (BBTV Bengaluru and BBTV Chikkaballapura) isolated from banana belonged to the Pacific Indian Ocean group. BSV isolate (Chickaballapura) isolated from banana in the present study was considered as a new variant of BSV. There is recombination in the genome DNA-1 of BBTV but no recombination in BSV. The molecular docking analysis for coat protein of four banana viruses (BBTV, BSV, Banana bract mosaic virus, cucumber mosaic virus) revealed that the terpenoids (Cucurbitacin-A, cucurbitacin-B, Musabalbisiane B, Musabalbisiane C, Musabalbisiane A), flavonoids (Vitexin, isovitexin, isoorientin, orientin, Isoscoparin, quercetin-3-o-glucoside, apigenin-7-0-glucoside, kaemferol-3-0-rhamnoside) and
antioxidants (Cucumerin-A and cucumerin-B) from phytochemicals and antiviral agents (Luotonin-A, streptindole, carrageenan, tylophorinine, antofine, ribavirin, peonidin, swertianolin) and Phytoalexins (Musanolone E, Musanolone F) were shown good binding affinities against coat protein of four banana viruses, which indicates the antiviral potentiality of phytochemicals and antiviral agents