STUDIES ON INTERSPECIFIC HYBRIDIZATION IN GENUS PRUNUS
Loading...
Date
2015
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
Abstract
ABSTRACT
The present investigations entitled “Studies on interspecific hybridization in genus Prunus” were conducted in Department of Fruit Science, Dr Y S Parmar University of Horticulture and Forestry, Nauni, Solan. Two late maturing high chill cultivars namely July Elberta, Redhaven and two low chill early maturing cultivars TropicBeauty, Partap and plum cv. Santa Rosa were included for controlled hybridization studies. Time and duration of flowering in cultivars studied varied a great deal. Duration of flowering extended from 16 to 30 days. Low chill peaches did not synchronise in flowering with plum cv. Santa Rosa. Fresh pollen viability ranged from 68.18 per cent with erythrosin B (0.04 %) in plum cv. Santa Rosa to 97.00 per cent with acetocarmine (2 %) in peach cv. July Elberta. Maximum in vitro pollen germination was obtained in 10 per cent sucrose after 48 hours to the extent of 86.50 per cent in peach cv. July Elberta and in plum cv. Santa Rosa, maximum in vitro pollen germination was obtained in 15 per cent sucrose after 48 hours (62.02%). Stored pollen viability was maximum (94.24%) in acetocarmine (2%) in peach cv. July Elberta. In plum cv. Santa Rosa too, maximum viability of stored pollen grains was 68.55 per cent in acetocarmine (2%). In vitro pollen germination was high in 10 per cent sucrose in peach cultivars but it was high for plum cv. Santa Rosa in 15 per cent sucrose. In vivo pollen germination occurred in first 24-48 hours of pollination but in vivo pollen tube growth took varied time ranging from 72 hours to 120 hours in different cross–combinations. The highest fruit set (71.84 %) was observed in cross combination ‘Partap × Santa Rosa’. Minimum fruit set (23.60 %) was recorded in cross combination ‘Redhaven × Santa Rosa’. Maximum fruits dropped in ‘Redhaven × Santa Rosa’ and minimum in Partap × Santa Rosa cross-combination. Maximum number of hybrid seeds obtained was 146 from cross- combination ‘July Elberta × Santa Rosa’ and minimum of 33 were recovered from ‘Partap × Santa Rosa’ cross-combination. Considerable success has been achieved via attempting inter-specific crosses as evident from moderate to high fruit set. The hybrid seeds so obtained comprise a valuable genepool which needs to be raised and tested for their hybridity and desirable horticultural traits.