ISOLATION, CHARACTERIZATION AND SCREENING OF PGPR FROM SALINE SOILS OF TELANGANA AND FIELD EVALUATION OF EFFICIENT ISOLATES ON GREENGRAM UNDER SALINE STRESS CONDITIONS
dc.contributor.advisor | TRIVENI, S | |
dc.contributor.author | BIYYANI SUMAN | |
dc.date.accessioned | 2020-10-03T05:30:26Z | |
dc.date.available | 2020-10-03T05:30:26Z | |
dc.date.issued | 2018 | |
dc.description.abstract | Salinity is one of the major abiotic stress limiting the productivity of agriculture in India, successful remediation of salt degraded areas for crop production is based on sustainable management practices which involves the use of salt tolerant microorganisms which are of low cost and easily adaptable method by the farmers. Present investigation was focussed towards isolation of salinity tolerant bacteria from the saline rhizospheric soils of Telangana and in vitro screening of the isolates for NaCl tolerance and field evaluation of the efficient isolates on greengram crop under saline stress conditions. Eighty salt tolerant bacteria were isolated from 20 villages of Mahabubnagar, Nalgonda and Rangareddy districts of Telangana, as the districts are prone to saline soils. The isolates were culturally, morphologically and biochemically characterized, among 80 isolates 35 were identified as Bacillus sp., 22 as Pseudomonas sp., 16 as Azotobacter sp., and 7 as Rhizobium sp. according to the Bergey’s manual of Systemic Bacteriology. The isolates were screened in vitro for salinity tolerance and 40 isolates which tolerated 15 per cent NaCl were further evaluated for drought tolerance, ACC deaminase activity, EPS production and plant growth promoting attributes like phosphorous, potassium and zinc solubilization, siderophore, HCN and IAA production. Results revealed that among the 40 isolates 42.5 per cent isolates showed tolerance at - 0.73 MPa, 22.5 per cent isolates utilized ACC as a sole nitrogen source, 55 per cent isolates produced EPS, phosphorous was solubilized by 40 per cent isolates, potassium by 22.5 per cent, zinc by 82.5 per cent isolates, siderophores was produced by 27.5 per cent isolates, HCN was produced by 70 per cent and IAA was produced by 50 per cent isolates. Six efficient isolates were selected and identified by 16S rRNA gene sequencing. BLAST search results through NCBI revealed 98 per cent similarity of the isolate PJ 21 with Bacillus licheniformis, 99 per cent similarity of PJ 23 with Pseudomonas syringae, 98 per cent similarity of PJ 37 with Pseudomonas fluorescens, 99 per cent similarity of PJ 19 with Pseudomonas alcaligenes, 97 per cent similarity of PJ 70 with Azotobacter sp. and 98 per cent similarity of PJ 71 with Bacillus subtilis. Two pot culture experiments were conducted during Kharif and Rabi, 2017. The results revealed the Rabi data was superior over Kharif data due to residual effect of microbial consortium which influenced crop production. Plant height at 60 DAS was found significantly highest in T9 with 28.67 cm in Rabi, fresh weight at 60 DAS was registered significantly highest in T9 with 14.33 g and dry weight was found significantly highest in the consortium treatment with 5.08 g. Total bacterial population was enumerated and found to be significantly highest in T9 (T1 + Seed treatment with consortium) with 4.28 x 107 , 7.42 x 107 and 5.82 x 107 CFU g-1 of soil at 20, 40 and 60 DAS respectively. Influence of salinity tolerant strains on nodulation was assessed and found highest in the consortium treated pots with 19.67 nodule number, 46.24 mg p-1 of nodule fresh weight and 38.87 mg p-1 of nodule dry weight. Influence on enzyme activity at flowering stage was assessed with the enzymes urease, dehydrogenase and phosphatase and found significantly highest in T9 with 32.6 µg NH4 N g-1 d -1 , 8.2 µg TPF g-1 d -1 and 128.4 µg of PNP g-1 h -1 activity respectively. Number of pods per plant was measured at harvest and found highest in seed treatment with consortium with 14.8, seed yield of plants was also found to be highest in T9 with 6.8 q ha-1 . Soil analysis was carried out for NPK at harvest and showed highest in consortium treatment with 2 - 3 per cent increase over control. In the field experiment, plant height, fresh weight and dry weight at 60 DAS was found significantly highest in T9 and increased two fold over control. Total bacterial population was enumerated and found to be significantly highest in T9 (T1 + Seed treatment with consortium) with 3 - 4 fold population over control. Influence of strains on nodulation was measured and consortium treated plants improved nodule number by 30 per cent, nodule fresh weight by 35 per cent and nodule dry weight by 45 per cent. Soil enzymes like urease, dehydrogenase and phosphatase activity was found significantly more in T9 with 35 per cent, 80 per cent and 45 per cent increase respectively over control. Number of pods per plant was measured at harvest and found highest in consortium treatment with two fold increase and seed yield of plants was found to increase by 47 per cent over control. Soil analysis was carried out for NPK at harvest and found 2 -3 fold increase of consortium treatment when compared with control. | en_US |
dc.identifier.citation | D10,350 | en_US |
dc.identifier.uri | https://krishikosh.egranth.ac.in/handle/1/5810152221 | |
dc.keywords | ISOLATION, CHARACTERIZATION AND SCREENING OF PGPR FROM SALINE SOILS OF TELANGANA AND FIELD EVALUATION OF EFFICIENT ISOLATES ON GREENGRAM UNDER SALINE STRESS CONDITIONS | en_US |
dc.language.iso | English | en_US |
dc.publisher | PROFESSOR JAYASHANKAR TELANGANA STATE AGRICULTURAL UNIVERSITY | en_US |
dc.sub | Agricultural Microbiology | en_US |
dc.theme | Ph.D | en_US |
dc.these.type | Ph.D | en_US |
dc.title | ISOLATION, CHARACTERIZATION AND SCREENING OF PGPR FROM SALINE SOILS OF TELANGANA AND FIELD EVALUATION OF EFFICIENT ISOLATES ON GREENGRAM UNDER SALINE STRESS CONDITIONS | en_US |
dc.type | Thesis | en_US |
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