STUDIES ON GENETIC STABILITY OF IN VITRO PROPAGATED CHRYSANTHEMUM CULTIVAR “PURNIMA” USING MOLECULAR MARKERS

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dc.contributor.advisorNATH, AMARJEET K.
dc.contributor.authorSHARMA, SHIKHA
dc.date.accessioned2016-11-28T12:57:20Z
dc.date.available2016-11-28T12:57:20Z
dc.date.issued2015
dc.description.abstractABSTRACT The present research entitled “Studies on genetic stability of in vitro propagated chrysanthemum cultivar “Purnima” using molecular markers.” was carried out during 20132014 & 2014-2015 in the Department of Biotechnology Nauni, Solan (H.P.) Dr. Y S Parmar University of Horticulture and Forestry, Nauni, Solan (H.P.). Axillary buds and leaf segments were used as explants for regeneration of plants. Chrysanthemum plants of the cultivar “Purnima”were regenerated from axillary bud by shoot bud proliferation and by an intermediate callus stage from leaves. The concentration of 2mg/l BAP + 0.5mg/l NAA and 1.5mg/l BAP + 0.25 mg/l NAA were found best for establishment of axillary buds and leaf explant. MS medium supplemented with 0.2mg/l BAP + 0.2mg/l NAA+1mg/l GA3 and 1 mg/l BAP + 0.5 mg/l NAA + 1mg/l GA3 concentration were found best for axillary bud proliferation and callus induction from leaf segments, respectively. MS medium supplemented with 1.0mg/l 2, 4-D +1.0 mg/l kinetin and 2mg/l BAP + 0.25mg/l NAA produced maximum number of shoots formed from axillary buds and from callus cultures, respectively. The rooting ½ strength MS medium containing 0.5mg/l IBA, produced maximum number of roots from both explants. Plant regenerated from axillary buds showed early flowering. The genetic stability between mother plant, plant regenerated from axillary bud and callus culture were carried out by using RAPD markers. Genomic DNA was isolated from young leaves of plants using CTAB method and amplified using 20 random decamer primers out of these only 15 produced polymorphism. Similarity coefficient was calculated by using Dices coefficient ranged from 0.30 to 0.50. Dendrogram was constructed by using UPGMA method for the clustering of mother plant, plants regenerated from axillary bud and callus. Mother plant formed separate cluster while plant regenerated from axillary bud and callus together formed one cluster that further subdivided. From the data obtained during present study it can be concluded that plants produced in vitro either by micropropagation or from callus culture were not genetically similar to mother plant.en_US
dc.identifier.other48332
dc.identifier.urihttp://krishikosh.egranth.ac.in/handle/1/87832
dc.language.isoenen_US
dc.subBiotechnology and Molecular Biology
dc.these.typeM.Sc
dc.titleSTUDIES ON GENETIC STABILITY OF IN VITRO PROPAGATED CHRYSANTHEMUM CULTIVAR “PURNIMA” USING MOLECULAR MARKERSen_US
dc.typeThesisen_US
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