SELECTION OF BACTERIAL BLIGHT (Xanthomonas axonopodis pv. punicae) RESISTANT SOURCE FROM INDIGENOUS WILD POPULATIONS OF Punica granatum L. (Daru) AND HYBRIDIZATION STUDIES
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Date
2023-11-04
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UHF,NAUNI
Abstract
ABSTRACT
The present investigation focussed on selecting bacterial blight-resistant sources from
indigenous wild populations of Punica granatum (Daru) and conducting hybridization studies.
The study involved surveying and collecting wild Daru germplasm, followed by screening the
collected genotypes for resistance against Xanthomonas axonopodis pv. punicae. Phylogenetic
analysis using SSR markers provided insights into the genetic relationships among the resistant
germplasm. The study also involved conducting attached leaf assays to evaluate the resistance of
genotypes under more realistic conditions. Several genotypes exhibited potential resistance, with
SH-14 and SH-16 showing the lowest disease severity and scores. Principal component analysis
and clustering helped group the genotypes based on variability and identified genotypes
conferring resistance. The genetic diversity studies using SSR markers revealed a high level of
polymorphism and unique bands in certain genotypes. Hybridization studies involved crossing
disease-resistant genotypes with commercial pomegranate cultivars. The cross Bhagwa × SH-16
showed the highest fruit set percentage, followed by Bhagwa × SL-23. Conversely, crosses
involving Kandhari Kabuli × SL-23, Kandhari Kabuli × SH-16, and other combinations had
lower fruit set percentages. Fruit retention percentages varied among different cross
combinations, with the highest recorded in Bhagwa × SL-23 and Kandhari Kabuli × SR-11, while
Phule Super Bhagwa × SH-16 had the lowest fruit retention. These findings contribute to the
identification of bacterial blight-resistant sources and provide insights for further breeding and
selection of pomegranate genotypes with improved resistance and yield potential. The future
research activities will focus on evaluating hybrid progenies, constructing genetic linkage maps,
and identifying specific genes associated with bacterial blight resistance in pomegranate. These
efforts aim to develop improved cultivars with enhanced resistance, leading to increased
productivity and decreased losses from the disease.