Production and partial purification of cellulases from Penicillium roqueforti NCIM 712 using poultry dropping based biodigested slurry
Loading...
Date
2017
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
Punjab Agricultural University, Ludhiana
Abstract
The present study was aimed at production and partial purification of cellulases from
Penicillium roqueforti NCIM 712 using poultry dropping based biodigested slurry. The
proximate and chemical analysis of biodigested slurry indicated the presence of sufficient
amount of cellulose for using it as a substrate for cellulase production. Cellulolytic enzyme
production was studied under autoclaved and unautoclaved conditions. Unautoclaved poultry
droppings based biodigested slurry showed higher activities of endoglucanase, exoglucanase
and β glucosidase. Optimized cultural conditions for cellulase production were inoculum size
of 107 spores/ml, 30°C of incubation temperature, 7 days of incubation period, pH of 5.0, and
slurry concentration of 100%, where 145.76 U/ml of endoglucanase, 46.46 U/ml of
exoglucanase and 11.61 U/ml of β glucosidase were obtained under unautoclaved conditions
and 62.91 U/ml of endoglucanase, 41.31 U/ml of exoglucanase and 9.98 U/ml of β
glucosidase were obtained under autoclaved conditions. The cellulases produced under
optimum conditions were partially purified by ion exchange chromatography. Two isoforms
were observed in endoglucanase i.e. Pool I and Pool II which showed 8.54 and 9.96 fold
purification respectively. The exoglucanase was purified by 11.45 folds and β glucosidase by
10.8 folds. The exoglucanase was further studied for its temperature and pH stability.
Exoglucanase exhibited maximum stability at 45°C and pH 5.0. Production of biogas was
studied from paddy straw supplemented with animal wastes including poultry droppings and
biodigested slurry. Highest biogas production (65.19 litres/ kg paddy straw) was observed in
soaked paddy straw supplemented with cellulolytic enzymes from P. roqueforti NCIM 712
produced in poultry droppings based biodigested slurry. There was 22% enhancement in
biogas produced from paddy straw mixed with enzymatically activated poultry droppings
biodigested slurry than paddy straw mixed with poultry droppings based biodigested slurry.
Hence poultry droppings based biodigested slurry was found to be suitable substrate for cost
effective cellulase enzyme production which can be further used for increasing biogas
production from paddy straw.
Description
Keywords
null