Production and partial purification of cellulases from Penicillium roqueforti NCIM 712 using poultry dropping based biodigested slurry

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dc.contributor.advisorPhutela, Urmila Gupta
dc.contributor.authorVersha Suman
dc.date.accessioned2018-01-17T13:37:13Z
dc.date.available2018-01-17T13:37:13Z
dc.date.issued2017
dc.description.abstractThe present study was aimed at production and partial purification of cellulases from Penicillium roqueforti NCIM 712 using poultry dropping based biodigested slurry. The proximate and chemical analysis of biodigested slurry indicated the presence of sufficient amount of cellulose for using it as a substrate for cellulase production. Cellulolytic enzyme production was studied under autoclaved and unautoclaved conditions. Unautoclaved poultry droppings based biodigested slurry showed higher activities of endoglucanase, exoglucanase and β glucosidase. Optimized cultural conditions for cellulase production were inoculum size of 107 spores/ml, 30°C of incubation temperature, 7 days of incubation period, pH of 5.0, and slurry concentration of 100%, where 145.76 U/ml of endoglucanase, 46.46 U/ml of exoglucanase and 11.61 U/ml of β glucosidase were obtained under unautoclaved conditions and 62.91 U/ml of endoglucanase, 41.31 U/ml of exoglucanase and 9.98 U/ml of β glucosidase were obtained under autoclaved conditions. The cellulases produced under optimum conditions were partially purified by ion exchange chromatography. Two isoforms were observed in endoglucanase i.e. Pool I and Pool II which showed 8.54 and 9.96 fold purification respectively. The exoglucanase was purified by 11.45 folds and β glucosidase by 10.8 folds. The exoglucanase was further studied for its temperature and pH stability. Exoglucanase exhibited maximum stability at 45°C and pH 5.0. Production of biogas was studied from paddy straw supplemented with animal wastes including poultry droppings and biodigested slurry. Highest biogas production (65.19 litres/ kg paddy straw) was observed in soaked paddy straw supplemented with cellulolytic enzymes from P. roqueforti NCIM 712 produced in poultry droppings based biodigested slurry. There was 22% enhancement in biogas produced from paddy straw mixed with enzymatically activated poultry droppings biodigested slurry than paddy straw mixed with poultry droppings based biodigested slurry. Hence poultry droppings based biodigested slurry was found to be suitable substrate for cost effective cellulase enzyme production which can be further used for increasing biogas production from paddy straw.en_US
dc.identifier.urihttp://krishikosh.egranth.ac.in/handle/1/5810039546
dc.keywordsBiodigested slurry, Biogas production, Cellulases, Optimization, P.roqueforti NCIM 712, Partial purification, Proximate and chemical compositionen_US
dc.language.isoenen_US
dc.pages88en_US
dc.publisherPunjab Agricultural University, Ludhianaen_US
dc.research.problemProduction and partial purification of cellulases from Penicillium roqueforti NCIM 712 using poultry dropping based biodigested slurryen_US
dc.subMicrobiologyen_US
dc.subjectnullen_US
dc.themeProduction and partial purification of cellulases from Penicillium roqueforti NCIM 712 using poultry dropping based biodigested slurryen_US
dc.these.typeM.Scen_US
dc.titleProduction and partial purification of cellulases from Penicillium roqueforti NCIM 712 using poultry dropping based biodigested slurryen_US
dc.typeThesisen_US
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