Linkage mapping of QTLs for grain iron content and evaluation of PAU201 x Palman 579 (iron rich) segregating population(s) in rice (Oryza sativa L.)

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dc.contributor.advisorJain, Rajinder Kumar
dc.contributor.authorWalia, Priyanka
dc.date.accessioned2017-06-03T03:29:44Z
dc.date.available2017-06-03T03:29:44Z
dc.date.issued2015
dc.description.abstractLinkage mapping and marker assisted selection can greatly accelerate the efficacy of breeding programs to improve mineral (iron and zinc) content in rice grains. A F2 population developed from the cross between PAU201 (high yielding indica) and Palman 579 (iron rich indica) was used to map the quantitative trait loci (QTL) associated with grain mineral content; the population consisted of 50 plants displaying wide variation in grain mineral content. As many as 82/327 polymorphic microsatellite (SSR) markers were used for linkage mapping. A total of 187 alleles and 18 new recombinant alleles (different that those in parent rice varieties) were identified. NTSYS-pc based analysis (cluster tree analysis, 2D-PCA and 3D-PCA) showed dispersion of the F2 population between the two distinct parental genotypes. Composite interval mapping (CIM) analysis by WinQTL cartographer 2.5 revealed a total of nineteen QTL for mineral content in grains on chromosome 2 and 10. Eight QTL for iron content were mapped on chromosome 2 (qFE1, qFE2, qFE3, qFE4, qFE5, qFE6, qFE7 and qFE8 at map positions 34.8, 54.2, 63.5, 70.0, 90.1, 152.1, 160.0 and 182.6 cM respectively) and four on chromosome 10 (qFE9, qFE10 qFE11 and qFE12 at map positions 19.4, 37.4, 54.9 and 72.8 cM respectively). Seven QTL for zinc content were mapped on chromosome 2 (qZN1, qZN2, qZn3 and qZN4 at map positions 88.3, 93.6, 152.5 and 158.5 cM respectively) and chromosome 10 (qZN5, qZN6 and qZN7, at map positions 36.2, 45.5 and 53.8 respectively). A notable aspect of this study is that QTL qFE5, qFE6 and qFE7 (90.1, 152.1 and 158.5 cM) for iron content and qZN1, qZN3 and qZN 4 (88.3, 152.5 and 158.5 cM) for zinc content on chromosome 2 are in close proximity, respectively, with the same flanking markers. Similarly, on chromosome 10, qFE10 and qFE11 (37.3 and 54.9 cM) are in close proximity of qZN5and qZN7 (36.2 and 53.8 cM), respectively, thus indicating that these QTL for iron and zinc content may in fact be linked. PAU201/Palman 579 F 3 and F 4 populations were evaluated for various agronomic traits and mineral content which displayed large variation for plant height (55.5 - 158.0 cm), effective number of tillers per plant (1 - 31), panicle length (15.2 - 30.6 cm), grain yield per plant (1.6 - 66.6 g), 1000 grain weight (10.5 - 33.3 g) and grain length/breadth ratio (2.72 - 4.83), Fe content (4.7 - 508.8 µg/g) and Zn content (1.2 - 130.4 µg/g) . Notably, nine F3 plants and eleven F 4 plants had iron content more than iron-rich donor parent, Palman 579. Grain iron content showed significant positive correlation (r=0.829 and 0.335) with grain zinc content in both F 3 and F 4 populations indicating the feasibility of improving Fe and Zn levels simultaneously in rice grains. As many as 50 promising F4 plants with grains rich in iron, zinc or both iron and zinc were selected for the future breeding programs.en_US
dc.identifier.urihttp://krishikosh.egranth.ac.in/handle/1/5810014308
dc.language.isoenen_US
dc.publisherCCSHAUen_US
dc.subBiotechnology and Molecular Biology
dc.subjectOryza sativa, Mineral, Microsatellite (SSR) markers, QTLen_US
dc.these.typePh.D
dc.titleLinkage mapping of QTLs for grain iron content and evaluation of PAU201 x Palman 579 (iron rich) segregating population(s) in rice (Oryza sativa L.)en_US
dc.typeThesisen_US
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