Molecular Characterization and Antimicrobial Sensitivity Pattern of Methicillin Resistant Staphylococcus aureus among Farm Animals
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Date
2021-10-18
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ANDUAT, Kumarganj, Ayodhya
Abstract
Isolation and characterization of methicillin resistant Staphylococcus aureus was
attempted from cattle, buffalo, sheep and goat. Processing of 375 nasal swab samples
(cattle 100, buffalo 100, sheep 100 and goat 75) and 350 milk samples (cattle 100,
buffalo 100, sheep 75 and goat 75) yielded 171 S. aureus isolates. All isolates were
catalase positive and majority of isolates gave positive slide coagulase (152), tube
coagulase test (143), fermented mannitol sugar (169), DNase test (142), β haemolysis
(157), growth on chromagar media plate (140) and latex agglutination test (139).
Out of 171 isolates 164 (95.91%) were resistant for methicillin, 158 (92.39%) for
cefoxitin, 168 (98.25%) for oxacillin and 163 (95.32%) for ampicillin by using disc
diffusion method. Highest number of isolates were susceptible for vancomycin (152,
88.89%) followed by ciprofloxacin (151, 88.30%), gentamycin (147, 85.96%),
tetracycline (128, 74.85%), erythromycin (126, 73.68%) and chloramphenicol (125,
73.10%). In E test 16 out of 171 isolates were found resistant against vancomycin.
In SDS-PAGE all the isolates irrespective of animal species and site of collected
sample revealed similar band pattern of protein in size (10 to 200 kDA molecular
weight) and numbers except nasal swab of buffalo and milk samples of sheep. Out of
171 methicillin resistant Staphylococcus aureus isolates recovered during this study,
mec A and fem A gene could be amplified in 137 and 133 isolates with an amplification
size of 533 and 510bp respectively. Amplified spa fragments varied in size, ranging
from 300 to 600 bp, depending on the spa type. The phylogenetic analysis showed four
major clusters. The MRSA isolates from nasal swab of goat i.e. SPA 7 were found to
be more distantly related to other isolates.
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