DETECTION AND MOLECULAR TYPING OF TOXINS OF Clostridium perfringens ISOLATES FROM SMALL RUMINANTS
| dc.contributor.advisor | SRIVANI, M(MAJOR) | |
| dc.contributor.advisor | ANAND KUMAR, P | |
| dc.contributor.advisor | ASWANI KUMAR, K | |
| dc.contributor.author | SAI MANI DEEPIKA, CHEDE | |
| dc.date.accessioned | 2023-06-03T12:17:26Z | |
| dc.date.available | 2023-06-03T12:17:26Z | |
| dc.date.issued | 2023-02 | |
| dc.description | THESIS | en_US |
| dc.description.abstract | A study was carried out on the isolation, biochemical, molecular characterization and toxin typing of C. perfringens isolated from healthy and diarrhoeic sheep and goat. The antibiotic resistance pattern was studied in representative samples. A total of 158 samples (rectal swabs and intestinal tissues) were collected from healthy and diarrhoeic sheep and goat, from which 34 (21.51%) C. perfringens isolates were obtained. A higher isolation rate was obtained from intestinal content samples (67.65%) than faecal samples (32.35%). The samples that exhibited characteristic cultural and biochemical characters were subjected to PCR, which revealed 34 samples were positive for C. perfringens. The sugars like galactose, cellobiose and raffinose were used to differentiate toxin type A, D and F from other toxin types, respectively. Out of 34 positive samples, a high prevalence rate was observed among diarrhoeic lambs (50.0%) followed by diarrhoeic sheep (44.12%) and healthy sheep (5.88%). Multiplex PCR for six toxin genes (alpha, beta, epsilon, iota, entero and beta2) of 34 isolates revealed the production of four toxins namely alpha, epsilon, entero and beta2 toxins belonging to three toxin types i.e., type A, D and F. Out of these isolates, 30 (88.23%) isolates were characterized as C. perfringens type A, one (2.94%) isolate was characterized as C. perfringens type D and three (8.82%) isolates were characterized as C. perfringens type F. In the present study, C. perfringens type A was found to be predominant among all the toxin types. Among diarrhoeic sheep, 86.67% and 13.33% of C. perfringens type A and type F were detected, respectively. The isolates obtained from healthy sheep were detected as type A and among diarrhoeic lambs, 88.24%, 5.88% and 5.88% of type A, D and F were detected, respectively. Antibiogram of randomly selected isolates showed resistance to sulphamethizole (66.67%) followed by co-trimoxazole (58.33%), enrofloxacin (41.67%) and tetracycline (33.33%) and high sensitivity observed against gentamicin (91.67%) followed by cefotaxime/clavulanic acid (91.67%), norfloxacin (91.67%) followed by chloramphenicol (83.33%), cefotaxime (75.0%) and streptomycin (66.67%). Sequencing results of selected isolates showed 100% homology with corresponding published toxin genes confirming the amplicons as C. perfringens (481 bp), alpha (324 bp) and epsilon (376 bp) toxin genes of C. perfringens. The present study concluded that the most of the isolates were characterized as C. perfringens type A. Therefore, it may be recommended to include C. perfringens type A anaculture in the existing vaccine, to prevent C. perfringens infections in this geographic region (Krishna and West Godavari districts of Andhra Pradesh). | en_US |
| dc.identifier.uri | https://krishikosh.egranth.ac.in/handle/1/5810197515 | |
| dc.keywords | DETECTION; MOLECULAR TYPING; TOXINS; Clostridium perfringens; ISOLATES; SMALL RUMINANTS | en_US |
| dc.language.iso | English | en_US |
| dc.pages | 114 | en_US |
| dc.publisher | SRI VENKATESWARA VETERINARY UNIVERSITY TIRUPATI - 517 502. (A.P.) INDIA | en_US |
| dc.sub | Veterinary Microbiology | en_US |
| dc.theme | DETECTION AND MOLECULAR TYPING OF TOXINS OF Clostridium perfringens ISOLATES FROM SMALL RUMINANTS | en_US |
| dc.these.type | M.V.Sc. | en_US |
| dc.title | DETECTION AND MOLECULAR TYPING OF TOXINS OF Clostridium perfringens ISOLATES FROM SMALL RUMINANTS | en_US |
| dc.type | Thesis | en_US |